論文

査読有り
2008年1月

Mannosylated semiconductor quantum dots for the labeling of macrophages

JOURNAL OF CONTROLLED RELEASE
  • Yuriko Higuchi
  • ,
  • Machiko Oka
  • ,
  • Shigeru Kawakami
  • ,
  • Mitsuru Hashida

125
2
開始ページ
131
終了ページ
136
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jconrel.2007.10.007
出版者・発行元
ELSEVIER SCIENCE BV

Quantum dots show strong fluorescence emission and long stability compared with classical organic fluorescent dyes; therefore, quantum dots take the place of other dyes in Western blot, immunostaining and bioimaging. Since macrophage plays crucial roles in many pathophysiological processes, tracking macrophage migration, homing and fate is important for understanding the complex roles of macrophages in disease or developing disease diagnosis. Because of the high expression of mannose receptor on macrophage, mannosylation is an attractive strategy to label macrophage. In this study, using polyethylene-glycol (PEG) (M.W. 2,000; PEG(2,000))-coated quantum dots, we prepared mannosylated PEG(2,000) (Man-PEG(2,000)) quantum dots for labeling macrophage. The uptake characteristics of Man-PEG(2,000) quantum dots were investigated by primary cultured peritoneal macrophages. The uptake of Man-PEG(2,000) quantum dots was inhibited by an excess amount of mannose, suggesting mannose receptor-mediated uptake of Man-PEG(2,000) quantum dots. The result of MTT assay suggested the extremely low cytotoxicity of Man-PEG(2,000) quantum dots. In conclusion, the Man-PEG(2,000) synthesized is safe and is taken up by macrophage mannose receptor recognition. (C) 2007 Elsevier B.V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.jconrel.2007.10.007
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902282606674594
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18045722
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000253069900007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.jconrel.2007.10.007
  • ISSN : 0168-3659
  • J-Global ID : 200902282606674594
  • PubMed ID : 18045722
  • Web of Science ID : WOS:000253069900007

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