2009年9月
Enhanced Gene Transfection in Macrophages by Histidine-Conjugated Mannosylated Cationic Liposomes
BIOLOGICAL & PHARMACEUTICAL BULLETIN
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- 巻
- 32
- 号
- 9
- 開始ページ
- 1628
- 終了ページ
- 1631
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1248/bpb.32.1628
- 出版者・発行元
- PHARMACEUTICAL SOC JAPAN
Targeted gene delivery to macrophages is important for the treatments of various immune diseases. Since macrophages express mannose receptors, development of efficient mannosylated non-viral carriers is an effective approach to macrophages-selective in vivo gene transfection. In this study, a pH-sensitive mannosylated cholesterol derivative, Man-His-C4-Chol, which possesses histidine (His) residues, containing lipoplexes (Man-His-lipoplexes) was characterized for transfection both in vitro and in vivo. In primary cultured macrophages, both Man-His-lipoplexes and mannosylated (Man)-lipoplexes showed significantly higher cellular uptake than bare-lipoplexes and there was no significant difference between Man-His-lipoplexes and Man-lipoplexes at 37 degrees C but the cellular uptake of these three lipoplexes was reduced at 4 degrees C. Similarly, the transfection efficacy of Man-His-lipoplexes showed significantly higher gene expression than bare-lipoplexes and Man-lipoplexes. After intraperitoneal administration to mice, Man-His-lipoplexes showed higher gene expression in peritoneally exuded cells (PECs) which contained macrophages than Man-lipoplexes and bare-lipoplexes at 3, 6, and 24h. In addition, Man-His-lipoplexes showed higher gene expression than Gal-His-lipoplexes in PECs, suggesting that Man-His-lipoplexes are taken up by macrophages via mannose receptor-mediated endocytosis. These results suggest that Man-His-lipoplexes have superior transfection activity to Man-lipoplexes in macrophages.
- リンク情報
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- DOI
- https://doi.org/10.1248/bpb.32.1628
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902273902352382
- CiNii Articles
- http://ci.nii.ac.jp/naid/130000117297
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19721246
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000269349000027&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1248/bpb.32.1628
- ISSN : 0918-6158
- J-Global ID : 200902273902352382
- CiNii Articles ID : 130000117297
- PubMed ID : 19721246
- Web of Science ID : WOS:000269349000027