2010年3月
gamma-Polyglutamic acid-coated vectors for effective and safe gene therapy
JOURNAL OF CONTROLLED RELEASE
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- 巻
- 142
- 号
- 3
- 開始ページ
- 404
- 終了ページ
- 410
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jconrel.2009.11.010
- 出版者・発行元
- ELSEVIER SCIENCE BV
In the present study, we developed some novel gene delivery vectors, coated cationic complexes with gamma-polyglutamic acid (gamma-PGA) for effective and safe gene therapy. Cationic complexes were constructed with pDNA and cationic vectors, such as poly-L-arginine hydrochloride (PLA), poly-L-lysine hydrobromide (PLL), N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride (DOTMA)-cholesterol (Chol) liposomes, and DOTMA-dioleylphosphatidylethanolamine (DOPE) liposomes. The cationic complexes showed high gene expression with strong cytotoxicity in melanoma B16-F10 cells. The cationic complexes were also strongly toxic to erythrocytes. On the other hand, the gamma-PGA was able to coat all cationic complexes and form stable nano-sized particles with negative charges. These gamma-PGA-coated complexes had high gene expression without cytotoxicity and toxicities to the erythrocytes. In in vivo transfection experiments, polyplexes showed high transfection efficiency over 10(5) RLU/g in the lung tissue after intravenous injection, although gamma-PGA-coated polyplexes showed a high value in the spleen. High transfection efficiency in lipoplexes and gamma-PGA-coated lipoplexes was observed in the spleen and lung. Thus, gamma-PGA-coated vectors are useful for clinical gene therapy. (C) 2009 Elsevier B.V. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.jconrel.2009.11.010
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201002260988408322
- CiNii Articles
- http://ci.nii.ac.jp/naid/120001759343
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19931327
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000276579500016&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.jconrel.2009.11.010
- ISSN : 0168-3659
- eISSN : 1873-4995
- J-Global ID : 201002260988408322
- CiNii Articles ID : 120001759343
- PubMed ID : 19931327
- Web of Science ID : WOS:000276579500016