2020年7月2日
Simple and Sensitive Analysis for Dried Blood Spot Proteins by Sodium Carbonate Precipitation for Clinical Proteomics
Journal of Proteome Research
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- 巻
- 19
- 号
- 7
- 開始ページ
- 2821
- 終了ページ
- 2827
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acs.jproteome.0c00271
Copyright © 2020 American Chemical Society. Dried blood spots (DBS) are widely used for screening biomolecular profiles, including enzymatic activities. However, detection of minor proteins in DBS by liquid chromatography-mass spectrometry (LC-MS/MS) without pre-enrichment remains challenging because of the coexistence of large quantities of hydrophilic proteins. In this study, we address this problem by developing a simple method using sodium carbonate precipitation (SCP). SCP enriches hydrophobic proteins from DBS, allowing substantial removal of soluble proteins. In combination with SCP, we used quantitative LC-MS/MS proteome analysis in a data-independent acquisition mode (DIA) to enhance the sensitivity and quantification limits of proteome analysis. As a result, identification of 1977 proteins in DBS is possible, including 585 disease-related proteins listed in the Online Mendelian Inheritance in Man.
- リンク情報
- ID情報
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- DOI : 10.1021/acs.jproteome.0c00271
- ISSN : 1535-3893
- eISSN : 1535-3907
- PubMed ID : 32343581
- SCOPUS ID : 85087469595