2019年
Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea
Journal of Tissue Engineering and Regenerative Medicine
- 巻
- 13
- 号
- 6
- 開始ページ
- 1019
- 終了ページ
- 1030
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/TERM.2849
Tracheal resection is often performed for malignant tumours, congenital anomalies, inflammatory lesions, and traumatic injuries. There is no consensus on the best approach for the restoration of tracheal functionality in patients with tracheal defects. Artificial grafts made of polypropylene and collagen sponge have been clinically used by our group. However, 2 months are required to achieve adequate epithelialization of the grafts in humans. This study aimed to investigate the feasibility of transplantation therapy using an artificial trachea with human-induced pluripotent stem cell (hiPSC)-derived multiciliated airway cells (hiPSC-MCACs). Collagen vitrigel membrane, a biocompatible and absorbable material, was used as a scaffold to cover the artificial trachea with hiPSC-MCACs. Analyses of hiPSC-MCACs on collagen vitrigel membrane were performed by immunocytochemistry and electron microscopy and by assessing ciliary beat frequency. Along with the artificial trachea, hiPSC-MCACs were transplanted into surgically created tracheal defects of immunodeficient rats. The survival of transplanted cells was histologically evaluated at 1 and 2 weeks after the transplantation. The hiPSC-MCACs exhibited motile cilia on collagen vitrigel membrane. The surviving hiPSC-MCACs were observed in the endotracheal epithelium of the tracheal defect at 1 and 2 weeks after transplantation. These results suggest that hiPSC-MCAC is a useful candidate for tracheal reconstruction.
- リンク情報
-
- DOI
- https://doi.org/10.1002/TERM.2849
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/30809958
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000473660800010&DestApp=WOS_CPL
- URL
- https://publons.com/publon/38235960/
- ID情報
-
- DOI : 10.1002/TERM.2849
- ISSN : 1932-6254
- ORCIDのPut Code : 97774698
- PubMed ID : 30809958
- Web of Science ID : WOS:000473660800010