NAKATSU Yoshimichi

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Name
NAKATSU Yoshimichi
Affiliation
Kyushu University
Section
Faculty of Medical Sciences
Job title
Associate Professor
Degree
Doctor of Science(Kyushu University)

Research Areas

 
 

Academic & Professional Experience

 
2000
 - 
2001
Associate Professor, Institute for Molecular and Cellular Biology, Osaka University.
 
1994
 - 
2000
Research Associate, Institute for Molecular and Cellular Biology, Osaka University.
 
1990
 - 
1994
Postdoctoral fellow, Fox Chase Cancer Center.
 
1988
 - 
1990
Research Associate, Kyushu University.
 
1985
 - 
1988
Postdoctoral fellow, Japan Society for the Promotion of Science.
 

Education

 
 
 - 
1985
Biology, Graduate School, Division of Natural Science, Kyushu University
 
 
 - 
1979
Biology, Faculty of Science, Kyushu University
 

Published Papers

 
Yusuke Matsuno, Yuko Atsumi, Atsuhiro Shimizu, Kotoe Katayama, Haruka Fujimori, Mai Hyodo, Yusuke Minakawa, Yoshimichi Nakatsu, Syuzo Kaneko, Ryuji Hamamoto, Teppei Shimamura, Satoru Miyano, Teruhisa Tsuzuki, Fumio Hanaoka, Ken ichi Yoshioka
Nature Communications   10    Dec 2019
© 2019, The Author(s). Mismatch repair (MMR)-deficient cancers are characterized by microsatellite instability (MSI) and hypermutation. However, it remains unclear how MSI and hypermutation arise and contribute to cancer development. Here, we show...
Genki Hayashida, Seijiro Shioi, Kyoko Hidaka, Ryosuke Fujikane, Masumi Hidaka, Toshiki Tsurimoto, Teruhisa Tsuzuki, Shinya Oda, Yoshimichi Nakatsu
Experimental Cell Research   377 24-35   Apr 2019
© 2019 The Authors Repeat destabilisation is variously associated with human disease. In neoplastic diseases, microsatellite instability (MSI) has been regarded as simply reflecting DNA mismatch repair (MMR) deficiency. However, several discrepan...
Yutaka Matsubara, Takuya Matsumoto, Keiji Yoshiya, Ayae Yoshida, Seiichi Ikeda, Tadashi Furuyama, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Masatoshi Nomura, Yoshihiko Maehara
Shock   51 364-371   Mar 2019
© 2019 Lippincott Williams and Wilkins. All rights reserved. Severe sepsis is critical to health and can result in acute renal failure (ARF). Tissue factor (TF) and thrombomodulin (TM) play key roles in vascular endothelial functions by helping ma...
Tetsuya Suzuki, Yuri Yanai, Natsuki Nishigaki, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Hiroyuki Kamiya
Journal of Bioscience and Bioengineering   125 619-623   May 2018
© 2017 The Society for Biotechnology, Japan The introduction of a 5′-tailed duplex (5′-TD) fragment into cells corrects a base-substitution mutation in a target DNA. We previously reported that the gene correction efficiency was improved when a fr...
Yoko Yamanishi, Ryotaro Tanaka, Yuta Arakawa, Yoshimichi Nakatsu
Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)   444-447   Feb 2017
© 2017 IEEE. We have succeeded in injection of plasmid to adherent cells which are suspended in the plasma-bubbles laden circulation flow in a chamber. High-speed plasma-bubbles are generated by glass electrode and the air-liquid interface has a s...
Issei Egashira, Fumi Takahashi-Yanaga, Risa Nishida, Masaki Arioka, Kazunobu Igawa, Katsuhiko Tomooka, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Yusaku Nakabeppu, Takanari Kitazono, Toshiyuki Sasaguri
Cancer Science   108 108-115   Jan 2017
© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. We previously reported that celecoxib, a selective COX-2 inhibitor, strongly inhibited human colon cancer cell proliferati...
Daisuke Matsuda, Takuya Matsumoto, Kenichi Honma, Ayae Ikawa-Yoshida, Mitsuho Onimaru, Tadashi Furuyama, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Yoshihiko Maehara
In Vivo   30 769-776   Nov 2016
© 2016, International Institute of Anticancer Research. All rights reserved. Background/Aim: Budding uninhibited by benzimidazole-related 1 (BUBR1) plays an important role in the spindle assembly checkpoint to prevent chromosome missegregation and...
Ayae Ikawa-Yoshida, Takuya Matsumoto, Shinji Okano, Yukihiko Aoyagi, Yutaka Matsubara, Tadashi Furuyama, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Mitsuho Onimaru, Tomoko Ohkusa, Masatoshi Nomura, Yoshihiko Maehara
Scientific Reports   6    Aug 2016
© The Author(s) 2016. A delay in liver regeneration after partial hepatectomy (PHx) leads to acute liver injury, and such delays are frequently observed in aged patients. BubR1 (budding uninhibited by benzimidazole-related 1) controls chromosome m...
Hiroyuki Kamiya, Natsuki Nishigaki, Akihiro Ikeda, Seiya Yukawa, Yukiko Morita, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Hideyoshi Harashima
Nucleosides, Nucleotides and Nucleic Acids   35 379-388   Jul 2016
© 2016 Taylor & Francis Group, LLC. A 5′-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined....
H. Yamamoto, M. Ishimura, M. Ochiai, H. Takada, K. Kusuhara, Y. Nakatsu, T. Tsuzuki, K. Mitani, T. Hara
Gene Therapy   23 205-213   Feb 2016
© 2016 Macmillan Publishers Limited. X-linked agammaglobulinemia (XLA) is one of the most common humoral immunodeficiencies, which is caused by mutations in Bruton's tyrosine kinase (BTK) gene. To examine the possibility of using gene therapy for ...
Rie Kanao, Masayuki Yokoi, Tsuyoshi Ohkumo, Yasutaka Sakurai, Kantaro Dotsu, Shinobu Kura, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Chikahide Masutani, Fumio Hanaoka
DNA Repair   29 139-146   May 2015
© 2015 Elsevier B.V. Xeroderma pigmentosum variant (XP-V) is a human rare inherited recessive disease, predisposed to sunlight-induced skin cancer, which is caused by deficiency in DNA polymerase η (Polη). Polη catalyzes accurate translesion synth...
Ryoichi Kyuragi, Takuya Matsumoto, Yui Harada, Satoru Saito, Mitsuho Onimaru, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Masatoshi Nomura, Yoshikazu Yonemitsu, Yoshihiko Maehara
Arteriosclerosis, Thrombosis, and Vascular Biology   35 341-347   Feb 2015
© 2014 American Heart Association, Inc. OBJECTIVE - : BubR1, a cell cycle-related protein, is an essential component of the spindle checkpoint that regulates cell division. Mice with BubR1 expression reduced to 10% of the normal level display a ph...
Naoya Kubokura, Fumi Takahashi-Yanaga, Masaki Arioka, Tatsuya Yoshihara, Kazunobu Igawa, Katsuhiko Tomooka, Sachio Morimoto, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Yusaku Nakabeppu, Takayuki Matsumoto, Takanari Kitazono, Toshiyuki Sasaguri
Journal of Pharmacological Sciences   127 446-455   Jan 2015
© 2015 The Authors. Differentiation-inducing factor-1 (DIF-1) produced by Dictyostelium discoideum strongly inhibits the proliferation of various types of cancer cells by suppression of the Wnt/β-catenin signal transduction pathway. In the present...
Takuro Isoda, Yoshimichi Nakatsu, Kazumi Yamauchi, Jingshu Piao, Takashi Yao, Hiroshi Honda, Yusaku Nakabeppu, Teruhisa Tsuzuki
International Journal of Biological Sciences   10 940-947   Aug 2014
MUTYH is a DNA glycosylase that excises adenine paired with 8-oxoguanine to prevent mutagenesis in mammals. Biallelic germline mutations of MUTYH have been found in patients predisposed to a recessive form of familial adenomatous polyposis (MAP: M...
Fumi Takahashi-Yanaga, Tatsuya Yoshihara, Kentaro Jingushi, Kazuhiro Igawa, Katsuhiko Tomooka, Yutaka Watanabe, Sachio Morimoto, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Yusaku Nakabeppu, Toshiyuki Sasaguri
Biochemical Pharmacology   89 340-348   Jun 2014
We reported that differentiation-inducing factor-1 (DIF-1), synthesized by Dictyostelium discoideum, inhibited proliferation of various tumor cell lines in vitro by suppressing the Wnt/β-catenin signaling pathway. However, it remained unexplored w...
Jingshu Piao, Yoshimichi Nakatsu, Mizuki Ohno, Ken ichi Taguchi, Teruhisa Tsuzuki
International journal of biological sciences   10 73-79   Jan 2013
We have previously established an experimental system for oxidative DNA damage-induced tumorigenesis in the small intestine of mice. To elucidate the roles of mismatch repair genes in the tumor suppression, we performed oxidative DNA damage-induce...
Teik How Lim, Ryosuke Fujikane, Shiori Sano, Ryuji Sakagami, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Mutsuo Sekiguchi, Masumi Hidaka
DNA Repair   11 259-266   Mar 2012
O 6-Methylguanine produced in DNA by the action of simple alkylating agents, such as N-methyl-N-nitrosourea (MNU), causes base-mispairing during DNA replication, thus leading to mutations and cancer. To prevent such outcomes, the cells carrying O ...
Riyoko Ito, Mutsuo Sekiguchi, Daiki Setoyama, Yoshimichi Nakatsu, Yuriko Yamagata, Hiroshi Hayakawa
Journal of Biochemistry   149 731-738   Jun 2011
MutT-related proteins, including Escherichia coli MutT and the human MTH1 (NUDT1), degrade 8-oxo-7, 8-dihydrodeoxyguanosine triphosphate (8-oxo-dGTP) to 8-oxo-dGMP and thereby prevent mutations caused by the misincorporation of 8-oxoguanine into D...
Teruhisa Tsuzuki, Jing Shu Piao, Takuro Isoda, Kinihiko Sakumi, Yusaku Nakabeppu, Yoshimichi Nakatsu
Health physics   100 293-294   Mar 2011
Hiroyuki Kamiya, Masayuki Uchiyama, Jingshu Piao, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Hideyoshi Harashima
International Journal of Pharmaceutics   387 180-183   Mar 2010
Targeted sequence alteration would be an attractive method in gene therapy and biotechnology. To achieve in vivo targeted sequence alteration, a tailed duplex DNA consisting of annealed 35mer and 794mer single-stranded DNAs was delivered by means ...
K. Komori, Y. Takagi, M. Sanada, T. H. Lim, Y. Nakatsu, T. Tsuzuki, M. Sekiguchi, M. Hidaka
Oncogene   28(8) 1142-1150   Feb 2009
O6-Methylguanine produced in DNA induces mutation due to its ambiguous base-pairing properties during DNA replication. To suppress such an outcome, organisms possess a mechanism to eliminate cells carrying O 6-methylguanine by inducing apoptosis t...
Hironobu Nakane, Seiichi Hirota, Philip J. Brooks, Yusaku Nakabeppu, Yoshimichi Nakatsu, Yoshitake Nishimune, Akihiro Iino, Kiyoji Tanaka
DNA Repair   7(12) 1938-1950   Dec 2008
We have reported that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] are deficient in nucleotide excision repair (NER) and highly sensitive to UV-induced skin carcinogenesis. Although xeroderma pigmentosum group A patients...
Hiroyuki Kamiya, Masayuki Uchiyama, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Hideyoshi Harashima
Journal of Biochemistry   144(4) 431-436   Oct 2008
The correction of an inactivated hygromycin resistance and enhanced green fluorescent protein (Hyg-EGFP) fusion gene by a several hundred-base single-stranded (ss) DNA fragment has been reported. In this study, the effectiveness of this type of ge...
Hiroyuki Tsuchiya, Masayuki Uchiyama, Kazuhiro Hara, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Hideo Inoue, Hideyoshi Harashima, Hiroyuki Kamiya
Biochemistry   47(33) 8754-8759   Aug 2008
A 606-base single-stranded (ss) DNA fragment, prepared by restriction enzyme digestion of ss phagemid DNA, corrects a hygromycin resistance and enhanced green fluorescent protein (Hyg-EGFP) fusion gene more efficiently than a PCR fragment, which i...
Masayuki Sanada, Masumi Hidaka, Yasumitsu Takagi, Tomoko Y. Takano, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Mutsuo Sekiguchi
Carcinogenesis   28(12) 2657-2663   Dec 2007
O6-Methylguanine and O6-chloroethylguanine, which are the primary cytotoxic DNA lesions produced by 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide (dacarbazine) and 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU...
Katsumi Sakamoto, Yohei Tominaga, Kazumi Yamauchi, Yoshimichi Nakatsu, Kunihiko Sakumi, Kaoru Yoshiyama, Akinori Egashira, Shinobu Kura, Takashi Yao, Masazumi Tsuneyoshi, Hisaji Maki, Yusaku Nakabeppu, Teruhisa Tsuzuki
Cancer Research   67(14) 6599-6604   Jul 2007
MUTYH is a mammalian DNA glycosylase that initiates base excision repair by excising adenine opposite 8-oxoguanine and 2-hydroxyadenine opposite guanine, thereby preventing G:C to T:A transversion caused by oxidative stress. Recently, biallelic ge...
Teruhisa Tsuzuki, Yoshimichi Nakatsu, Yusaku Nakabeppu
Cancer Science   98(4) 465-470   Apr 2007
Reactive oxygen species (ROS) are produced through normal cellular metabolism, and their formation is further enhanced by exposure to ionizing radiation and various chemicals. ROS attack DNA, and the resulting oxidative DNA damage is considered to...
Isao Kuraoka, Shinsuke Ito, Tadashi Wada, Mika Hayashida, Lily Lee, Masafumi Saijo, Yoshimichi Nakatsu, Megumi Matsumoto, Tsukasa Matsunaga, Hiroshi Handa, Jun Qin, Yoshihiro Nakatani, Kiyoji Tanaka
Journal of Biological Chemistry   283(2) 940-950   2007
Nucleotide excision repair is a versatile repair pathway that counteracts the deleterious effects of various DNA lesions. In nucleotide excision repair, there is a transcription-coupled repair (TCR) pathway that focuses on DNA damage that blocks R...
Yusaku Nakabeppu, Kunihiko Sakumi, Katsumi Sakamoto, Daisuke Tsuchimoto, Teruhisa Tsuzuki, Yoshimichi Nakatsu
Biological chemistry   387(4) 373-379   Jan 2006
Genomes and their precursor nucleotides are highly exposed to reactive oxygen species, which are generated both as byproducts of oxygen respiration or molecular executors in the host defense, and by environmental exposure to ionizing radiation and...
Rie Yonemasu, Mitsuyoshi Minami, Yoshimichi Nakatsu, Masayo Takeuchi, Isao Kuraoka, Yoichi Matsuda, Yujiro Higashi, Hisato Kondoh, Kiyoji Tanaka
DNA Repair   4(4) 479-491   Apr 2005
The XAB2 protein (XPA-binding protein 2) with 15 tetratricopeptide repeat motifs has been isolated by virtue of its ability to interact with xeroderma pigmentosum group A (XPA) protein in the yeast two-hybrid system. It has been shown that XAB2 in...
Yan Ren, Masafumi Saijo, Yoshimichi Nakatsu, Hiroshi Nakai, Masaru Yamaizumi, Kiyoji Tanaka
Genes and Genetic Systems   78(1) 93-102   Feb 2003
Cockayne syndrome (CS) is a rare autosomal recessive disease, which shows diverse clinical symptoms such as photosensitivity, severe mental retardation and developmental defects. CS cells are hypersensitive to killing by UV-irradiation and defecti...
Masafumi Yoshino, Yoshimichi Nakatsu, Hein te Riele, Seiichi Hirota, Yukihiko Kitamura, Kiyoji Tanaka
DNA Repair   1(11) 935-940   Nov 2002
We have made xeroderma pigmentosum group A gene (XPA)-knockout mice (XPA-/- mice). The XPA-/- mice had no detectable activity for nucleotide excision repair (NER) and showed a high incidence of UVB-induced skin tumorigenesis. We have also found th...
Machiko Murai, Yasushi Enokido, Naoko Inamura, Masafumi Yoshino, Yoshimichi Nakatsu, Gijsbertus T.J. Van Der Horst, Jan H.J. Hoeijmakers, Kiyoji Tanaka, Hiroshi Hatanaka
Proceedings of the National Academy of Sciences of the United States of America   98(23) 13379-13384   Nov 2001
Xeroderma pigmentosum (XP) and Cockayne syndrome (CS) are rare autosomal recessive disorders associated with a defect in the nucleotide excision repair (NER) pathway required for the removal of DNA damage induced by UV light and distorting chemica...
Kiyoji Tanaka, Shinya Kamiuchi, Yan Ren, Rie Yonemasu, Minoru Ichikawa, Hiroaki Murai, Masafumi Yoshino, Seiji Takeuchi, Masafumi Saijo, Yoshimichi Nakatsu, Hiroko Miyauchi-Hashimoto, Takeshi Horio
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis   477(1/2) 31-40   Jun 2001
Nucleotide excision repair (NER) removes a wide variety of lesions from the genome and is deficient in the genetic disorder, xeroderma pigmentosum (XP). In this paper, an in vitro analysis of the XP group A gene product (XPA protein) is reported. ...
Yoshimichi Nakatsu, Hiroshi Asahina, Elisabetta Citterio, Suzanne Rademakers, Wim Vermeulen, Shinya Kamiuchi, Jing Ping Yeo, Min Cheh Khaw, Masafumi Saijo, Naohiko Kodo, Toshiro Matsuda, Jan H.J. Hoeijmakers, Kiyoji Tanaka
Journal of Biological Chemistry   275(45) 34931-34937   Nov 2000
Nucleotide excision repair is a highly versatile DNA repair system responsible for elimination of a wide variety of lesions from the genome. It is comprised of two subpathways: transcription-coupled repair that accomplishes efficient removal of da...
Masahiko Nitta, Masafumi Saijo, Naohiko Kodo, Toshiro Matsuda, Yoshimichi Nakatsu, Hiroshi Tamai, Kiyoji Tanaka
Nucleic Acids Research   28(21) 4212-4218   Nov 2000
The xeroderma pigmentosum group A protein (XPA) plays a central role in nucleotide excision repair (NER). To identify proteins that bind to XPA, we screened a HeLa cDNA library using the yeast two-hybrid system. Here we report a novel cytoplasmic ...
Minoru Ichikawa, Hironobu Nakane, Giancarlo Marra, Chantal Corti, Josef Jiricny, Maureen Fitch, James M. Ford, Miyoko Ikejima, Takashi Shimada, Masafumi Yoshino, Seiji Takeuchi, Yoshimichi Nakatsu, Kiyoji Tanaka
Mutation Research - DNA Repair   459(4) 285-298   May 2000
Xeroderma pigmentosum group A gene (XPA)-deficient mice are defective in nucleotide excision repair (NER) and are therefore highly sensitive to ultraviolet (UV)-induced skin carcinogenesis. We established cell lines from skin cancers of UVB-irradi...
Hiroaki Murai, Seiji Takeuchi, Yoshimichi Nakatsu, Minoru Ichikawa, Masafumi Yoshino, Yoichi Gondo, Motoya Katsuki, Kiyoji Tanaka
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis   450(1/2) 181-192   May 2000
We have established xeroderma pigmentosum group A (XPA) gene-knockout mice with nucleotide excision repair (NER) deficiency, which rapidly developed skin tumors when exposed to a low dose of chronic UV like XP-A patients, confirming that the NER p...
Takehiro Kobayashi, Seiji Takeuchi, Masafumi Saijo, Yoshimichi Nakatsu, Hiroshi Morioka, Eiko Otsuka, Mitsuo Wakasugi, Osamu Nikaido, Kiyoji Tanaka
Nucleic Acids Research   26(20) 4662-4668   Oct 1998
To analyze the function of the xeroderma pigmentosum group A (XPA) protein in strand-specific DNA repair, we examined repair of UV-induced cyclobutane pyrimidine dimer (CPD) in transcribed and non-transcribed strands of the dihydrofolate reductase...
Anne L. Lehman, Yoshimichi Nakatsu, Ada Ching, Roderick T. Bronson, Rebecca J. Oakey, Natalie Keiper-Hrynko, Joshua N. Finger, Donna Durham-Pierre, Daniel B. Horton, J. M. Newton, Mary F. Lyon, Murray H. Brilliant
Proceedings of the National Academy of Sciences of the United States of America   95(16) 9436-9441   Aug 1998
Three radiation-induced alleles of the mouse p locus, p(6H), p(25H), and p(bs), cause defects in growth, coordination, fertility, and maternal behavior in addition to p gene-related hypopigmentation. These alleles are associated with disruption of...
Y. Jing Ping, Y. Nakatsu, A. M. Goldstein, M. A. Tucker, K. H. Kraemer, K. Tanaka
Melanoma Research   8(1) 47-52   Mar 1998
Although some cases of dysplastic naevi (DN) and familial melanoma have been linked to anonymous markers on chromosome 1p36, the gene has not been identified. A candidate gene, RPA2, which codes for the 32 kDa subunit of replication protein A, is ...
Seiji Takeuchi, Yoshimichi Nakatsu, Hironobu Nakane, Hiroaki Murai, Seiichi Hirota, Yukihiko Kitamura, Akihiko Okuyama, Kiyoji Tanaka
Cancer Research   58(4) 641-646   Feb 1998
We examined the spectrum of p53 mutations found in 40 UV-induced skin tumors of xeroderma pigmentosum group A gene (XPA)-deficient mice. p53 mutations were detected in 48% of the tumors. Nearly all of the mutations were induced at dipyrimidine sit...
Takehiro Kobayashi, Isao Kuraoka, Masafumi Saijo, Yoshimichi Nakatsu, Akemi Tanaka, Yukiko Someda, Shuhei Fukuro, Kiyoji Tanaka
Human Mutation   9(4) 322-31   Apr 1997
XP is sun-sensitive and cancer-prone genetic disorder, consisting of eight (group A-G) genetically distinct complementation groups. Some XP group D patients exhibit clinical symptoms of other genetic disorders, CS, and TTD. The XP group D gene (XP...
Enokido Y, Inamura N, Araki T, Satoh T, Nakane H, Yoshino M, Nakatsu Y, Tanaka K, Hatanaka H.
J Neurochem.   69(1) 246-251   1997
Hironobu Nakane, Seiji Takeuchi, Shunsuke Yuba, Masafumi Saijo, Yoshimichi Nakatsu, Hiroaki Murai, Yoko Nakatsuru, Takatoshi Ishikawa, Seiichi Hirota, Yukihiko Kitamura, Yoko Kato, Yukio Tsunoda, Hiroko Miyauchi, Takeshi Horio, Tomoyuki Tokunaga, Tsukasa Matsunaga, Osamu Nikaido, Yoshitake Nishimune, Yoshio Okada, Kiyoji Tanaka
Nature   377(6545) 165-168   Sep 1995
XERODERMA pigmentosum (XP) is an autosomal recessive disorder characterized by a high frequency of skin cancer on sun-exposed areas, and neurological complications. XP has a defect in the early step(s) of nucleotide-excision repair (NER) and consi...
Akira Nagai, Masafumi Saijo, Isao Kuraoka, Toshiro Matsuda, Naohiko Kodo, Yoshimichi Nakatsu, Takashi Mimaki, Makoio Mino, Maureen Biggerstaff, Richard D. Wood, Anneke Sijbers, Jan H.J. Hoeijmakers, Kiyoji Tanaka
Biochemical and Biophysical Research Communications   211(3) 960-966   Jun 1995
The human XPA and ERCC1 proteins, which are involved in early steps of nucleotide excision repair of DNA, specifically interacted in an in vitro binding assay and a yeast two-hybrid assay. A stretch of consecutive glutamic acid residues in XPA was...
V. Greger, J. H.M. Knoll, E. Woolf, K. Glatt, R. F. Tyndale, T. M. Delorey, R. W. Olsen, A. J. Tobin, J. M. Sikela, Y. Nakatsu, M. H. Brilliant, P. J. Whiting, M. Lalande
Genomics   26(2) 258-264   Mar 1995
GABAA receptors are heterooligomeric ligand-gated ion channels that mediate the effect of the inhibitory neurotransmitter γ-aminobutyric acid. The GABAA receptors consist of at least 15 different receptor subunits that can be classified into 5 sub...
T. Matsuda, M. Saijo, I. Kuraoka, T. Kobayashi, Y. Nakatsu, A. Nagai, T. Enjoji, C. Masutani, K. Sugasawa, F. Hanaoka, A. Yasui, K. Tanaka
Journal of Biological Chemistry   270(8) 4152-4157   Jan 1995
XPA is a zinc finger DNA-binding protein, which is missing or altered in group A xeroderma pigmentosum cells and known to be involved in the damage- recognition step of the nucleotide excision repair (NER) processes. Using the yeast two-hybrid sys...
Susana Rosemblat, Donna Durham-Pierre, John M. Gardner, Yoshimichi Nakatsu, Murray H. Brilliant, Seth J. Orlow
Proceedings of the National Academy of Sciences of the United States of America   91(25) 12071-12075   Dec 1994
The pink-eyed dilution (p) locus in the mouse is critical to melanogenesis; mutations in the homologous locus in humans, P, are a cause of type II oculocutaneous albinism. Although a cDNA encoded by the p gene has recently been identified, nothing...
D. Durham-Pierre, J. M. Gardner, Y. Nakatsu, R. A. King, U. Francke, A. Ching, R. Aquaron, V. Del Marmol, M. H. Brilliant
Nature Genetics   7(2) 176-179   Jun 1994
Oculocutaneous albinism (OCA) is a genetically heterogeneous hypopigmentation disorder. One of the two major autosomal recessive forms involves the tyrosinase gene (OCA1), while the other form (OCA2) has recently been associated with alterations o...
MURRAY H. BRILLIANT, RICHARD KING, UTA FRANCKE, SIMONE SCHUFFENHAUER, THOMAS MEITINGER, JOHN M. GARDNER, DONNA DURHAM‐PIERRE, YOSHIMICHI NAKATSU
Pigment Cell Research   7(6) 398-402   Jan 1994
Mutations at the mouse pink‐eyed dilution locus, p, cause hypopigmentation. We have cloned the mouse p gene cDNA and the cDNA of its human counterpart, P. The region of mouse chromosome 7 containing the p locus is syntenic with human chromosome 15...
M. H. Brilliant, A. Ching, Y. Nakatsu, E. M. Eicher
Genetics   138(1) 203-211   Jan 1994
Allelic variation of the mouse pink-eyed dilution (p) gene in common laboratory strains and wild mice was examined by Southern blot and by polymerase chain reaction. In these assays the original p mutation allele found in strains SJL/J, 129/J, B10...
Yoshimichi Nakatsu, Rachel F. Tyndale, Timothy M. Delorey, Donna Durham-Pierre, John M. Gardner, Heather J. McDanel, Q. Nguyen, Joseph Wagstaff, Marc Lalando, James M. Sikela, Richard W. Olsent, Allan J. Tobin, Murray H. Brilliant
Nature   364(6436) 448-450   Jan 1993
THE mouse pink-eyed cleft-palate (pcp ) mutation is characterized by hypopigmentation associated with cleft palate, neurological disorders and runting1,2. Most pcp homozygotes are born with cleft palate and die shortly after birth, presumably as a...
Yoichi Gondo, John M. Gardner, Yoshimichi Nakatsu, Donna Durham-Pierre, Susan A. Deveau, Cynthia Kuper, Murray H. Brilliant
Proceedings of the National Academy of Sciences of the United States of America   90(1) 297-301   Jan 1993
The mouse pink-eyed unstable (pun) mutation, affecting coat color, exhibits one of the highest reported reversion frequencies of any mammalian mutation and is associated with a duplication of genomic DNA at the p locus. In this study, genomic clon...
Yoshimichi Nakatsu, Kazue Hattori, Hiroshi Hayakawa, Kenji Shimizu, Mutsuo Sekiguchi
Mutation Research-DNA Repair   293 119-132   Jan 1993
O 6 -Methylguanine-DNA methyltransferase plays an important role in cellular defence against mutagens and carcinogens with alkylating activity. Certain tumor-derived cell lines, termed Mer - , are defective in the enzyme activity and have an incre...
Chueh Ling-ling, Takanori Nakamura, Yoshimichi Nakatsu, Kunihiko Sakumi, Hiroshi Hayakawa, Mutsuo Sekiguchi
Carcinogenesis   13(5) 837-843   May 1992
O6-Methylguanine-DNA methyltransferase plays an important role in preventing tumor induction. To elucidate the significance of a highly conserved amino acid sequence of methyltransferase protein, amino acid substitutions were introduced by site-di...
Akiko Shiraishi, Kunihiko Sakumi, Yoshimichi Nakatsu, Hiroshi Hayakawa, Mutsuo Sekiguchi
Carcinogenesis   13(2) 289-296   Feb 1992
An enzyme, O6-methylguanine-DNA methyltransferase, is present in various organisms and plays an important role in repair of DNA damaged by alkylating agents. The enzyme transfers methyl groups from O6-methylguanine and other methylated moieties of...
John M. Gardner, Yoshimichi Nakatsu, Yoichi Gondo, Susan Lee, Mary F. Lyon, Richard A. King, Murray H. Brilliant
Science   257(5073) 1121-1124   Jan 1992
Complementary DNA clones from the pink-eyed dilution (p) locus of mouse chromosome 7 were isolated from murine melanoma and melanocyte libraries. The transcript from this gene is missing or altered in six independent mutant alleles of the p locus,...
Mary F. Lyon, Thomas R. King, Yoichi Gondo, John M. Gardner, Yoshimichi Nakatsu, Eva M. Elcher, Murray H. Brilliant
Proceedings of the National Academy of Sciences of the United States of America   89(15) 6968-6972   Jan 1992
Recessive mutant alleles at the pink-eyed dilution (p) locus on mouse chromosome 7 reduce pigmentation of both the coat and eyes. Here we describe the properties and complementation interactions of 10 p alleles, including 6 not previously reported...
Yoshimichi Nakatsu, Yoichi Gondo, Murray H. Brilliant
Mammalian Genome   2 69-71   Jan 1991
Y. Nakatsu, S. Nomoto, M. Oh-Uchida, K. Shimizu, M. Sekiguchi
Cold Spring Harbor Symposia on Quantitative Biology   51(Part2) 1001-1008   Dec 1986
K. Shimizu, Y. Nakatsu, M. Sekiguchi, K. Hokamura, K. Tanaka, M. Terada, T. Sugimura
Proceedings of the National Academy of Sciences of the United States of America   82(17) 5641-5645   Dec 1985
Transfection with high molecular weight DNA from a primary stomach cancer induced foci of transformed NIH 3T3 cells, and the transformed cells were tumorigenic in nude mice. By screening with a human Alu-family probe, we isolated the human DNA seq...
Yoshimichi Nakatsu, Yusaku Nakabeppu, Mutsuo Sekiguchi
Journal of Biochemistry   91(6) 2057-2065   Jan 1982
[3H]Thymine-labeled poly(dT) carrying many apyrimidinic (AP) sites has been prepared by treating an enzymatically synthesized poly(dA) poly(dT, dU) with uracil-DNA glycosylase. Incubation of the polymer with a homogeneous preparation of T4 endonuc...
Kenji Oeda, Yoshimichi Nakatsu, Mutsuo Sekiguchi
Journal of Biochemistry   87(5) 1387-1394   Jan 1980
Treatment of ØX174 replicative form (RF) DNA, pre-exposed to ultraviolet light, with T4 endonuclease V led to a marked increase of infectivity of the RF when the activity was assayed on CaCl2-treated cells of Escherichia coli strain defective in u...

Misc

 
Teruhisa Tsuzuki, Jing Shu Piao, Yoshimichi Nakatsu
Nihon rinsho. Japanese journal of clinical medicine   69 Suppl 3 137-142   Apr 2011
Yoshimichi Nakatsu, Mutsuo Sekiguchi
Oxidative Stress, Disease and Cancer   221-252   Jan 2006
Errol C. Friedberg, Fumio Hanaoka, Kiyoji Tanaka, Samuel H. Wilson, Akira Yasui, Kaoru Sugasawa, Tsukasa Matsunaga, Kiran Madura, Kevin Sweder, Bennett Van Houten, Priscilla Cooper, Tadahiro Shiomi, Arthur Grollman, Yusaku Nakabeppu, Susan Wallace, Masashi Takao, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Shinya Oda, Shinichi Fukushige, Roger Woodgate, Zhigang Wang, Chikahide Masutani, Haruo Ohmori, Eiji Ohashi, Thomas Kunkel, Myron F. Goodman, Takehiko Nohmi, Eiichiro Sonoda, Masaru Yamaizumi, Mutsuo Sekiguchi, Graham Walker, Hisaji Maki, Kazuo Yamamoto, Kaoru Yoshiyama, Isao Kuraoka, Kenshi Komatsu, Alan Tomkinson, Hideo Shinagawa, John Tainer, Helfrid Hochegger, Takemi Enomoto, Roger Schultz, Akira Shimamoto, Vilhelm Bohr
DNA Repair   2 639-652   May 2003
Nakatsu Y, Nomoto S, Oh-uchida M, Shimizu K, Sekiguchi M.
Cold Spring Harb Symp Quant Biol.   17(Part 2) 85-91   Jan 1986
We previously isolated a novel human transforming gene from a primary stomach cancer and identified it as an activated version of the c-raf-1 gene which is the human homologue of v-raf, a viral oncogene encoding a serine/threonine-specific protein...
Y. Nakatsu
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   46(8 Suppl) 908-915   Jan 2001
Yasushi Enokido, Naoko Inamura, Toshiyuki Araki, Takumi Satoh, Hironobu Nakane, Masafumi Yoshino, Yoshimichi Nakatsu, Kiyoji Tanaka, Hiroshi Hatanaka
Journal of Neurochemistry   69 246-251   Jul 1997
To study the involvement of the xeroderma pigmentosum group A gene (XPA) in neuronal apoptosis, we cultured cerebellar neurons from mice lacking XPA gene (XPA(-/-)) and induced apoptosis by exposure to UV irradiation or medium containing a low con...
Kiyoji Tanaka, Yoshimichi Nakatsu, Masafumi Saijo, Takehiro Kobayashi, Toshiro Matsuda, Isao Kuraoka, Hiroaki Murai, Hironobu Nakane, Seiji Takeuchi, Naohiko Kodo, Masahiko Nitta
Japanese Journal of Human Genetics   41 50   Dec 1996
Nucleotide excision repair (NER) is a versatile repair system capable of rectifying a broad spectrum of DNA damage. The importance of NER has been suggested by studies of the human inherited disease, xeroderma pigmentosum (XP), which is characteri...
Organization and expression of the human gene for O6-methylguanine-DNA methyltransferase.
Nakatsu Y, Hattori K, Hayakawa H, Shimizu K, Sekiguchi M.
Mutat Res.   263(2) 119-132   1993
The p locus is closely linked to the mouse homolog of a gene from the Prader-Willi chromosomal region.
Nakatsu Y, Gondo Y, Brilliant MH.
Mamm Genome.   2(1) 69-71   1992
K. Shimizu, Y. Nakatsu, M. Oh-uchida, S. Nomoto, M. Sekiguchi
Japanese Journal of Cancer and Chemotherapy   14(6) 2140-2146   Jun 1987
We previously isolated a novel human transforming sequence from a primary stomach cancer and identified the gene as an activated version of the c-raf-1 gene which is the human homologue of v-raf, a viral oncogene encoding a serine/threonine-specif...
K. Shimizu, Y. Nakatsu, M. Ohuchida, M. Sekiguchi
Japanese Journal of Human Genetics   30 93-94   Jan 1985

Books etc

 
"Oxidative Damage to Nucleotide: Consequences and Preventive" in "MechanismsOxidative Stress, Disease and Cancer"
Imperial College Press   2006   

Conference Activities & Talks

 
Identification of a novel apoptosis-inducing gene, Mapo1, using a method of retrovirus-mediated gene-trap mutagenesis
3R Symposium 2008   2008   
Potassium Bromate-Induced Tumorigenesis in the Small Intestine of Various DNA Repair-Deficient Mice
3R Symposium 2008   2008   
Oxidative Stress-Induced Tumorigenesis in the Small Intestine of Various DNA Repair-Deficient Mice
10th International Symposium on Organic Free Radicals (ISOFR 10) and 3rd Pacific Symposium on Radical Chemistry (PSRC3)   2008   
Oxidative Stress-Induced Tumorigenesis in the Small Intestine of Various DNA Repair-Deficient Mice
10th International Workshop on Radiation Damage to DNA (IWRDD)   2008   
Distinct pathways of apoptosis induced by alkylated DNA damage
The International Ataxia-Telangiectasia Workshop 2008   2008   

Works

 
Mutagenesis in DNA repair deficient mice.
1995 - 2001
Analysis of the genes involed in tanscription-coupled repair.
1996 - 2000
Tumorigenesis in DNA repair deficient mice.
2001 - 2002
Fundamental research for gene therapy.
2002

Research Grants & Projects

 
Avoidance mechanisms of oxidative damage in DNA and RNA.
Project Year: 2001 - 2004
Apoptosis caused by oxidative damages in DNA, RNA and Nucleotide pool.
Mutagenesis in mice.
Creation of Gene-Kockout and transgenic mice.