論文

査読有り
2017年2月

Laboratory-scale production of (S)-reticuline, an important intermediate of benzylisoquinoline alkaloids, using a bacterial-based method

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Eitaro Matsumura
  • ,
  • Akira Nakagawa
  • ,
  • Yusuke Tomabechi
  • ,
  • Takashi Koyanagi
  • ,
  • Hidehiko Kumagai
  • ,
  • Kenji Yamamoto
  • ,
  • Takane Katayama
  • ,
  • Fumihiko Sato
  • ,
  • Hiromichi Minami

81
2
開始ページ
396
終了ページ
402
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2016.1243985
出版者・発行元
TAYLOR & FRANCIS LTD

Benzylisoquinoline alkaloids (BIAs) are a group of plant secondary metabolites that have been identified as targets for drug discovery because of their diverse pharmaceutical activities. Well-known BIAs are relatively abundant in plants and have therefore been extensively studied. However, although unknown BIAs are also thought to have valuable activities, they are difficult to obtain because the raw materials are present at low abundance in nature. We have previously reported the fermentative production of an important intermediate (S)-reticuline from dopamine using Escherichia coli. However, the yield is typically limited. Here, we improved production efficiency by combining in vivo tetrahydropapaveroline production in E. coli with in vitro enzymatic synthesis of (S)-reticuline. Finally, 593mg of pure (S)-reticuline was obtained from 1 L of the reaction mixture. Because this bacterial-based method is simple, it could be widely used for production of (S)-reticuline and related BIAs, thereby facilitating studies of BIAs for drug discovery.

リンク情報
DOI
https://doi.org/10.1080/09168451.2016.1243985
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27740901
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000393294200027&DestApp=WOS_CPL
ID情報
  • DOI : 10.1080/09168451.2016.1243985
  • ISSN : 0916-8451
  • eISSN : 1347-6947
  • PubMed ID : 27740901
  • Web of Science ID : WOS:000393294200027

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