2009年5月
Hyperproduction of 3,4-Dihydroxyphenyl-L-alanine (L-Dopa) Using Erwinia herbicola Cells Carrying a Mutant Transcriptional Regulator TyrR
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 73
- 号
- 5
- 開始ページ
- 1221
- 終了ページ
- 1223
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.90019
- 出版者・発行元
- TAYLOR & FRANCIS LTD
In the last few decades, enzymatic production of 3,4-dihydroxyphenyl-L-alanine (L-dopa) using tyrosine phenol-lyase (Tpl) has been industrialized. This method has an intrinsic problem of tyrosine contamination because Tpl is synthesized under tyrosine-induced conditions. Herein, we constructed a hyper-L-dopa-producing strain by exploiting a mutant TyrR, an activator of tpl. The highest productivity was obtained for the strain grown under non-induced conditions. It was 30-fold higher than that obtained for tyrosine-induced wild-type cells.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.90019
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 19420686
- Web of Science ID : WOS:000266620100046