Shigehiro Yoshimura

Profile Information

Affiliation: -, Graduate School of Biostudies, Kyoto University

Degree: Ph.D(Kyoto University)

J-GLOBAL ID: 200901047703088958
researchmap Member ID: 5000032057

External link: http://www.lif.kyoto-u.ac.jp/labs/chrom

Research Interests

Research Areas

Research History

Apr, 2007 - Present

Associate professor, Biostudies, Graduate School, Kyoto University

Papers

104

Yes-associated protein regulates cortical actin architecture and dynamics through intracellular translocation of Rho GTPase-activating protein 18

Y.Zhang,T.Kitagawa, M.Furutani-Seiki, S.H.Yoshimura

The FASEB Journal. 2023;37:e23161. DOI:10.1096/fj.202201992R, Aug, 2023 Peer-reviewedCorresponding author
Self-assembly of CIP4 drives actin-mediated asymmetric pit-closing in clathrin-mediated endocytosis

Y.Yiming, S.H.Yoshimura

nature communications DOI:10.1038/s41467-023-40390-y, 2023 Peer-reviewedCorresponding author
Mechanisms of length-dependent recognition of viral double-standed RNA by RIG-I

J.H.Im, I.Duic, S.H.Yoshimura, K.Onomoto, M.Yoneyama, H.Kato, T.Fujita

Scientific Reports.13(1):6318. DOI:10.1038/s41598-023-33208-w., 2023 Peer-reviewed
Multidimensional fractal scaling analysis using higher order moving average polynomials and its fast algorithm

H.Ju, N.Honda, S.H.Yoshimura, M.Kaneko, T.Shigematsu, K.Kiyono

J.Signal Processing, 208,108997. DOI:10.2139/ssrn.4290825., 2023 Peer-reviewed
Nuclear import receptors are recruited by FG-nucleoporins to rescue hallmarks of TDP-43 proteinopathy

B.Khalil, D.Chhangani, M.C.Wren, C.L.Smith, J.H.Lee, X.Li,C.Puttinger, C.Tsai, G.Fortin, D.Morderer, J.Gao, F.Liu …

Mol.Neurodeg.,17(1):80. DOI:10.1186/s13024-022-00585-1, 2022 Peer-reviewed
Cell cycle-specific phase separation regulated by protein charge blockiness

H.Yamazaki, M.Takagi, H.Kosako, T.Hirano, S.H.Yoshimura

Nature Cell Biology.24(5):625-632. DOI:10.1038/s41556-022-00903-1., 2022 Peer-reviewedCorresponding author
Modulation of actin-binding and -bundling activities of MISP/Caprice by multiple phosphorylation

N.D.Maarof, M.Kumeta, S.H.Yoshimura

Biochem Biophys Res Commun. 561:128-135. DOI:10.1016/j.bbrc.2021.05.041., 2021 Peer-reviewedCorresponding author
High cell density increases glioblastoma cell viability under glucose deprivation via degradation of the cystine/glutamate transporter xCT (SLC7A11)

I.Yamaguchi, S.H.Yoshimura, H.Katoh

Journal of Biological Chemistry., 295(20):6936-6945, DOI:10.1074/jbc.RA119.012213., 2020 Peer-reviewed
Viral RNA recognition by LGP2 and MDA5, and activation of signaling through step-by-step conformational changes

I.Duic, H.Tadakuma, Y.Harada, R.Yamaue, K.Deguchi, Y.Suzuki, S.H.Yoshimura, H.Kato, K.Takeyasu, T.Fujita

Nucleic Acids Research, 48(20):11664-11674. DOI:10.1093/nar/gakka935., 2020 Peer-reviewed
Redox-sensitive cysteines confer proximal control of the molecular crowding barrier in the nuclear pore

W.Zhang, R.Watanabe, H. A. Konishi, T.Fujiwara, S.H. Yoshimura, M.Kumeta

Cell Reports. 33(11):108484. DOI:10.1016/j.celrep.2020.108484., 2020 Peer-reviewed
Interactions between non‐structured domains of FG‐ and non‐FG‐nucleoporins coordinate the ordered assembly of the nuclear pore complex in mitosis

H.A. Konishi, S.H.Yoshimura

The FASEB Journal.,34(1):1532-1545, DOI:10.1096/fj.201901669R., 2020 Peer-reviewedCorresponding author
Quantitative proteomics indicate a strong correlation of mitotic phospho-/dephosphorylation with non-structured regions of substrates

H.Yamazaki, H.Kosako, S.H.Yoshimura

Biochim. Biophys. Acta. Proteins and Proteom., 1868(1):140295, DOI:10.1016/j.bbapap.2019.140295., Jan, 2020 Peer-reviewedCorresponding author
発がん微小環境におけるDNA修復欠損の役割細胞膜張力制御と染色体分配(Defective DNA damage repair in cancer microenvironment Down-regulating cortical tension ensures timely chromosome segregation)

河野恵子, 西村耕太郎, 城村由和, 出口確, Jiang Zixian, 内田和彦, 鈴木成実, 嶋田緑, 千葉祥恵, 広田亨 …

日本癌学会総会記事, 78回 S2-5, Sep, 2019
Mark1 regulates distal airspace expansion through pneumocyte flattening in lung development.

K.Fumoto, H.Takigawa-Imamura, K.Sumiyama, S.H. Yoshimura,N.Maehara, A.Kikuchi

Journal of Cell Science., 132(24):jcs235556, DOI:10.1242/jcs.23556., 2019 Peer-reviewed
Cdk1-mediated DIAPH1 phosphorylation maintains cortical tension during metaphase, which regulates inactivation of the spindle assembly checkpoint at anaphase onset

K.Nishimura, Y.Johmura, K.Deguchi, Z.Jiang, K.S.K.Uchida, N.Suzuki, M.Shimada, Y. Chiba, T.Hirota, S.H.Yoshimura …

Nature communications.,10(1):981, DOI:10.1038/s41467-019-08957-w., 2019 Peer-reviewed
Morphological changes of plasma membrane and protein assembly during clathrin-mediated endocytosis.

A.Yoshida, N.Sakai, Y.Uekusa, Y.Imaoka, Y.Itagaki, Y.Suzuki, S.H.Yoshimura

PLOS biology.16(5):e2004786, DOI:10.1371/journal.pbio.2004786., 2018 Peer-reviewedCorresponding author
Cell type-specific suppression of mechanosensitive genes by audible sound stimulation.

M.Kumeta, D.Takahashi, K.Takeyasu, S.H.Yoshimura

PLOS One, 13(1): e0188764, DOI:10.1371/journal.pone.0188764., 2018 Peer-reviewed
N-terminal dual lipidation-coupled targeting into the primary cilium

M.Kumeta, Y.Panina, H.Yamazaki, K.Takeyasu, S.H.Yoshimura

Genes Cells, 23(8):715-723, DOI:10.1111/gtc.12603., 2018 Peer-reviewedCorresponding author
Prolines in the α-helix confer the structural flexibility and functional integrity of importin β.

M.Kumeta, H.A.Konishi, W.Zhang, S.Sakagami, S.H.Yoshimura

Journal of Cell Science,131(1):jcs206326, DOI:10.1242/jcs.206326, 2018 Peer-reviewedCorresponding author
In vivo dynamics of the cortical actin network revealed by fast-scanning atomic force microscopy.

Y.Zhang, A.Yoshida, N.Sakai, Y.Uekusa, M.Kumeta, S.H.Yoshimura

Microscopy, 66(4):272-282, DOI:10.1093/jmicro/dfx015., 2017 Peer-reviewedCorresponding author

Misc.

【印刷中】タンパク質のリン酸化は、なぜ非構造領域に群がるのか？～リン酸化が生む出す「電荷ブロック」型の液－液相分離制御～、

吉村成弘

2023 Peer-reviewedCorresponding author
【印刷中】電荷ブロック型液－液相分離の仕組みとリン酸化による制御、生物物理

山﨑啓也, 吉村成弘

2023 Peer-reviewed
タンパク質クラウディング状態の蛍光プローブによる測定

吉村成弘

実験医学別冊相分離解析プロトコール, 146-153, 2022 Peer-reviewedCorresponding author
核膜孔複合体の構造と機能における液－液相分離の役割

吉村成弘

実験医学 39（10）1527-1533., 2021 Peer-reviewedCorresponding author
核膜孔を介した分子輸送機構の構造的理解

吉村成弘

遺伝子医学 11(4)87-95., 2021 Peer-reviewedCorresponding author
How do flaviviruses hijack host cell functions by phase separation?

A.Saito, M.Shofa, H.Ode, M.Yumiya.j.Hirano,T, Okamoto, S.H.Yoshimura

Viruses, 13(8):1479, DOI:10.3390/v13081479., 2021 Peer-reviewedCorresponding author
Investigating the morphological dynamics of the plasma membrane by high-speed atomic force microscopy.

Y.Yu, S.H.Yoshimura

J.Cell Sci. 134(17): jcs243584, DOI:10.1242/jcs.243584., 2021 Peer-reviewedCorresponding author
細胞が情報物質を取り込む瞬間の撮影に成功ー細胞表層で進行する生命現象の理解と解明への技術革新

吉田藍子, 鈴木勇輝, 吉村成弘

化学, 74(2) 42-46, Feb, 2019 Invited
核膜孔複合体

吉村成弘

膜タンパク質高額ハンドブック, 2019
Down-regulating cortical tension ensures timely chromosome segregation

河野恵子, 西村耕太郎, 城村由和, 出口確, JIANG Zixian, 内田和彦, 鈴木成実, 嶋田緑, 千葉祥恵, 広田亨 …

日本癌学会学術総会抄録集(Web), 78th, 2019
高速AFMによるエンドサイトーシス膜変形過程のライブセルイメージング

吉田藍子, 鈴木勇輝, 吉村成弘

バイオサイエンスとインダストリー, 77(1) 48-50, Jan, 2019 Invited
高速原子間力顕微鏡で生きた細胞表面の動態を見る

吉田藍子, 鈴木勇輝, 吉村成弘

バイオサイエンスとインダストリー・目で見るバイオ, 77(1) 8-9, Jan, 2019 Invited
Involvement of Actin Dynamics in Endocytotic Process Revealed by Fast-Scanning Atomic Force Microscope

A.Yoshida, N.Sakai, Y.Uekusa, Y.Zhang, M.Kumeta, S.H.Yoshimura

BIOPHYSICAL JOURNAL, 112(3) 397A-397A, Feb, 2017
Non-Invasive Imaging of Membrane Dynamics Accompanied with Endocytosis in Living Cells by Atomic Force Microscopy

A.Yoshida, S.Ito, M.Kumeta, S.H.Yoshimura

BIOPHYSICAL JOURNAL, 110(3) 594A-594A, Feb, 2016
HEAT repeats－versatile arrays of amphiphilic helices working in crowded environments?

S.H.Yoshimura, T.Hirano

J.Cell Sci. 129(21):3963-3970, DOI:10.1242/jcs.185710., 2016 Peer-reviewed
Prolines in the alpha-helix confer structural flexibility on importin beta necessary for nuclear transport.

M.Kumeta, H.Konishi, S.H.Yoshimura

MOLECULAR BIOLOGY OF THE CELL, 27, 2016
Ran

吉村成弘

生体の科学, 2015
核膜孔複合体内部構造とインポーティンβ依存的物質輸送の分子基盤の理解

小西秀明, 吉村成弘

生化学 87（1）56-63., 2015 Peer-reviewed
Dissecting in vivo steady-state dynamics of karyopherin-dependent nuclear transport

O.Lolodi, H.Yamazaki, S.Otsuka, M.Kumeta, S.H.Yoshimura

MOLECULAR BIOLOGY OF THE CELL, 26, 2015
Nucleocytoplasmic shuttling of cytoskeletal proteins: molecular mechanism and biological significance.

M.Kumata, S.H.Yoshimura, J.Hejna, K.Takeyasu

Int. J. Cell Biol. 494902, DOI:10.1155/2012/494902., 2012 Peer-reviewed

Books and Other Publications

大学で学ぶ身近な生物学

吉村成弘

羊土社, Oct, 2015

Presentations

翻訳後修飾による液-液相分離制御機構の解明

吉村成弘

徳島大学酵素学研究セミナー（広島,日本）, Mar 2, 2023 Invited
リン酸化による細胞内液-液相分離制御機構

吉村成弘

定量生物学の会第10回年会（広島,日本）, Dec 15, 2022 Invited
翻訳後修飾による液‐液相分離制御機構の解明

吉村成弘

第45回日本分子生物学会年会（幕張,日本）, Dec 2, 2022 Invited
翻訳後修飾による液-液相分離制御機構

吉村成弘

日本プロテオーム学会 2022年大会（相模原,日本）, Aug 8, 2022 Invited
翻訳後修飾による液-液相分離制御機構の解明

吉村成弘

LLPS研究会（オンライン開催）, Jun 25, 2022 Invited
ライブセル高速原子間力顕微鏡による細胞表層骨格の可視化とメカノセンシング機構の解明

吉村成弘

第59回日本生体医工学会大会（オンライン開催）, May 26, 2020 Invited
How Clathrin-mediated Endocytosis Proceeds under Membrane Tension.

S.H.Yoshimura

International Symposium on AMED ”Mechanobiology” Project（Nagoya,Japan), Feb 8, 2020 Invited
液相分離による核・染色体の構造構築とリン酸化による制御

吉村成弘

よこはま NMR 研究会（横浜,日本）, Jan 16, 2020 Invited
Promiscuous interactions between non-structured domains of nucleoporins coordinate the ordered assembly of the nuclear pore complex in mitosis.

H.Konishi, H.Yamazaki, S.H.Yoshimura

第42回日本分子生物学会年会（福岡,日本）, Dec 5, 2019 Invited
Promiscuous interactions between non-structured domains of nucleoporins coordinate the ordered assembly of the nuclear pore complex in mitosis.

H.Konishi, H.Yamazaki, S.H.Yoshimura

Sep 18, 2019 Invited
Protein-induced morphological changes of the plasma membrane during clathrin-mediated endocytosis and its dependency on the membrane tension revealed by live-cell fast-scanning atomic force microscopy.

S.H.Yoshimura

EMBO workshop, Physics and Chemistry of Endocytosis （Ischia, Italy）, Sep 2, 2019 Invited
In vivo dynamics of the cortical actin network revealed by fast-scanning atomic force microscopy.

Y,Zhang,A.Yoshida, N.Sakai,Y, Uekusa, M.Kumeta, S.H.Yoshimura

Jun 18, 2019 Invited
Imaging morphological changes of the plasma membrane and protein assembly during clathrin-mediated endocytosis.

S.H.Yoshimura

FAOPS2019（Kobe,Japan）, Mar 29, 2019 Invited
ライブセル高速原子間力顕微鏡によるエンドサイトーシス膜変形過程の可視化解析

吉田藍子, 鈴木勇輝, 吉村成弘

第128回日本解剖学会学術集会（新潟,日本）, Mar 28, 2019 Invited
Non-invasive Live-cell Imaging of Plasma Membrane and Cortical Actin Dynamics in Endocytic Process by High-speed Atomic Force Microscopy.

A.Yoshida, Y.Itagaki, Y.Suzuki, S.H.Yoshimura

Nov 3, 2018 Invited
Live-cell analysis of actin network by high-speed atomic force microscopy.

Y.Itagaki, A.Yoshida, M.Kumeta, S.H.Yoshimura

16th International Student Seminar（Kyoto,Japan）, Feb 28, 2018 Invited
A nanoimaging approach for identification of the secondary structural domains in long ssRNA molecules.

J.L.Gilmore, A.Yoshida, H.Aizaki, M.Nakano, T.Wakita, S.H.Yoshimura, K.Takeyasu, T,Noda

62nd Annual Meeting of Biophysical Society （San Francisco, USA）, Feb 19, 2018 Invited
Live-cell imaging by high-speed atomic force microscopy revealed protein and plasma membrane dynamics during endocytic process.

S.H.Yoshimura

EMBO/EMBL Symposium：Seeing Believing－Imaging the process of Life（Heidelberg,Germany）, Oct 6, 2017 Invited
Live-cell Imaging of actin dynamics in cortex and lamellipodium by high-speed atomic force microscopy.

Y.Itagaki, Y.Zhang,A, Yoshida,N,Sakai, Y.Uekusa, M.Kumeta, S.H.Yoshimura

Sep 20, 2017 Invited
Morphological Analysis of clathrin-mediated endocytotic process by fast-scanning atomic force microscope

A.Yoshida, Y.Zhang, Y.Itagaki, M.Kumeta, Y.Suzuki, N.Sakai, Y.Uekusa, S.H.Yoshimura

Mar 17, 2017 Invited

Professional Memberships

Apr, 2018 - Present

日本顕微鏡学会
Apr, 2014 - Present

日本生物物理学会

Research Projects

Mechanical self-transformation of living systems

Grants-in-Aid for Scientific Research, Grant-in-Aid for Transformative Research Areas (A), Japan Society for the Promotion of Science, Jun, 2022 - Mar, 2027
Development of force-measuring system for biological tissues

Grants-in-Aid for Scientific Research, Grant-in-Aid for Transformative Research Areas (A), Japan Society for the Promotion of Science, 2022 - 2026
新規抗HIV-1宿主因子の抗ウイルス作用メカニズム解明と動物モデル開発への応用

エイズ対策実用化研究事業, 国立研究開発法人日本医療研究開発機構(AMED), Apr, 2022 - Mar, 2025

齊藤暁, 吉村成弘, 岡村智崇
live-cell hybrid imaging of morphological dynamics of the plasma membrane

Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Japan Society for the Promotion of Science, Oct, 2018 - Mar, 2022

YOSHIMURA Shigehiro
Global regulatory mechanism of endocytosis revealed by cell-surface imaging technique

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2022

Yoshimura Shigehiro
Analyses of cortical actin dynamics by live-cell imaging and machine-learning

Grants-in-Aid for Scientific Research, Challenging Research (Exploratory), Japan Society for the Promotion of Science, Jun, 2019 - Mar, 2021

Yoshimura Shigehiro
Functional analyses of host cell cortex in budding process of human immunodeficiency virus

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2021
フラビウイルスの生活環に特異的な抗フラビウイルス薬の創出

国立研究開発法人日本医療研究開発機構(AMED), Apr, 2020
ウイルス蛋白質の非構造領域による液相制御の解明と治療への応用

国立研究開発法人日本医療研究開発機構(AMED), Apr, 2020
倒立型顕微鏡一体型ＡＦＭ装置（ＢＩＸＡＭ）を用いた試料観察研究（平成30年度分）

オリンパス株式会社, Apr, 2018 - Mar, 2019
細胞膜動態の高分解計測と膜動態を標的とした創薬

キャノン財団研究助成「産業基盤の創生」, 2018 - 2019
原子間力顕微鏡を用いたコンデンシン、コヒーシン複合体の分子構造イメージング

学校法人沖縄科学技術大学院大学学園, Apr, 2017 - Mar, 2018
倒立型顕微鏡一体型ＡＦＭ装置（ＢＩＸＡＭ）を用いた試料観察研究（平成29年度分）

オリンパス株式会社, Apr, 2017 - Mar, 2018
Spatiotemporal imaging of membrane morphology and protein localization during endocytic process

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2018

Shigehiro Yoshimura, ADACHI Taiji
ＡＦＭを用いた試料観察研究（平成28年度分）

オリンパス株式会社, Apr, 2016 - Mar, 2017
低侵襲細胞表層イメージングによる細胞の内因性力発生・維持システムの動作原理解明

国立研究開発法人日本医療研究開発機構(AMED), Oct, 2016
Research for development of science curriculum for excellence: Toward fostering innovation in Japan

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2016

GINSHIMA Fumi, AOYAMA Kazuhiro, KIMURA Suteo, HATOGAI Taro
Spying minority in biological phenomena -Toward bridging dynamics between individual and ensemble processes-

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2011 - Mar, 2016

Nagai Takeharu, UEDA HIROK, ISHIJIMA AKIHIKO, IMADA KATSUMI, MAESHIMA KAZUHIRO, NOJI HIROYUKI, TOGASHI YUICHI, ARAI YOSHIYUKI, MATSUDA TOMOKI, YOSHIMURA SHIGEHIRO, HORIKAWA KAZUKI, WATANABE TOMONOBU, FUJITA KATSUMASA, KINBARA KAZUSHI, SANDO SHINSUKE, TAKEUCHI SHOJI, KOMATSUZAKI TAMIKI, OKADA YASUSHI, HARADA YOSHIE
Small number issues in the genome biology

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2011 - Mar, 2016

Maeshima Kazuhiro, TANIGUCHI YUUICHI, TAKAHASHI KOUICHI, YOSHIMURA NARIHIRO
AFMを用いた試料観察研究（平成27年度分）

オリンパス株式会社, Apr, 2015 - Mar, 2016

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