<p>Assessment of in vivo articular contact behavior at the wrist joint are important for understanding the biomechanical functions under physiological and pathological loading. The objective of this study was to evaluate in vivo articular contact behavior between the radius and scaphoid and between the radius and lunate using a two-dimensional to three-dimensional image matching technique. Two normal male subjects were enrolled in this study. Biplanar X-ray images were taken during dosal flexion/palmar flexion and ulnar/radial deviation of the wrist joint. The articular contact was estimated from the joint space distance smaller than the cartilage layer thickness measured using a MRI scanner. The distributions of estimated contact area and the contact locations varied markedly with wrist position. The present result would be able to provide a fundamental knowledge about the biomechanics of the wrist joint.</p>