MISC

2010年

Microfluorometric assay for quantification of anti-erythrocytic antibody level in sera of Plasmodium berghei ANKA infected semi-immune mice

Tropical Medicine and Health
  • Gideon K. Helegbe
  • ,
  • Nguyen T. Huy
  • ,
  • Tetsuo Yanagi
  • ,
  • Mohammed N. Shuaibu
  • ,
  • Mihoko Kikuchi
  • ,
  • Michio Yasunami
  • ,
  • Kenji Hirayama

38
4
開始ページ
127
終了ページ
131
記述言語
英語
掲載種別
DOI
10.2149/tmh.2010-13

A simple, sensitive and reproducible fluorometry method was developed to quantify anti-erythrocytic auto-antibody in the sera of semi-immune mice. The level of anti-erythrocytic auto-antibody measured using fluorometry was significantly higher in sera of Plasmodium berghei ANKA infected mice than the uninfected, p&lt
0.0001. This result correlates significantly with the standard ELISA assay, p&lt
0.0001, r2=0.7766. Both methods show significant correlation with regard to extent of Hb loss, p&lt
0.006 (r2=0.33) (fluorometry method) and p&lt
0.0001, r2=0.30 (ELISA). Thus the fluorometry method may serve as an alternative to standard ELISA assay and can be modified to suit similar objectives. © 2010 by The Japanese Society of Tropical Medicine.

リンク情報
DOI
https://doi.org/10.2149/tmh.2010-13
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79955030874&origin=inward
ID情報
  • DOI : 10.2149/tmh.2010-13
  • ISSN : 1348-8945
  • ISSN : 1349-4147
  • SCOPUS ID : 79955030874

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