2010
Microfluorometric assay for quantification of anti-erythrocytic antibody level in sera of Plasmodium berghei ANKA infected semi-immune mice
Tropical Medicine and Health
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- Volume
- 38
- Number
- 4
- First page
- 127
- Last page
- 131
- Language
- English
- Publishing type
- DOI
- 10.2149/tmh.2010-13
A simple, sensitive and reproducible fluorometry method was developed to quantify anti-erythrocytic auto-antibody in the sera of semi-immune mice. The level of anti-erythrocytic auto-antibody measured using fluorometry was significantly higher in sera of Plasmodium berghei ANKA infected mice than the uninfected, p<
0.0001. This result correlates significantly with the standard ELISA assay, p<
0.0001, r2=0.7766. Both methods show significant correlation with regard to extent of Hb loss, p<
0.006 (r2=0.33) (fluorometry method) and p<
0.0001, r2=0.30 (ELISA). Thus the fluorometry method may serve as an alternative to standard ELISA assay and can be modified to suit similar objectives. © 2010 by The Japanese Society of Tropical Medicine.
0.0001. This result correlates significantly with the standard ELISA assay, p<
0.0001, r2=0.7766. Both methods show significant correlation with regard to extent of Hb loss, p<
0.006 (r2=0.33) (fluorometry method) and p<
0.0001, r2=0.30 (ELISA). Thus the fluorometry method may serve as an alternative to standard ELISA assay and can be modified to suit similar objectives. © 2010 by The Japanese Society of Tropical Medicine.
- Link information
- ID information
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- DOI : 10.2149/tmh.2010-13
- ISSN : 1348-8945
- ISSN : 1349-4147
- eISSN : 1349-4147
- SCOPUS ID : 79955030874