2020年9月
Barthelonids represent a deep-branching metamonad clade with mitochondrion-related organelles predicted to generate no ATP
Proceedings of the Royal Society B
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- 巻
- 287
- 号
- 1934
- 開始ページ
- 20201538
- 終了ページ
- 20201538
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1098/rspb.2020.1538
- 出版者・発行元
- ROYAL SOC
We here report the phylogenetic position of barthelonids, small anaerobic flagellates previously examined using light microscopy alone. spp. were isolated from geographically distinct regions and we established five laboratory strains. Transcriptomic data generated from one strain (PAP020) were used for large-scale, multi-gene phylogenetic (phylogenomic) analyses. Our analyses robustly placed strain PAP020 at the base of the Fornicata clade, indicating that barthelonids represent a deep-branching metamonad clade. Considering the anaerobic/microaerophilic nature of barthelonids and preliminary electron microscopy observations on strain PAP020, we suspected that barthelonids possess functionally and structurally reduced mitochondria (i.e. mitochondrion-related organelles or MROs). The metabolic pathways localized in the MRO of strain PAP020 were predicted based on its transcriptomic data and compared with those in the MROs of fornicates. We here propose that strain PAP020 is incapable of generating ATP in the MRO, as no mitochondrial/MRO enzymes involved in substrate-level phosphorylation were detected. Instead, we detected a putative cytosolic ATP-generating enzyme (acetyl-CoA syn
- リンク情報
- ID情報
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- DOI : 10.1098/rspb.2020.1538
- ISSN : 0962-8452
- PubMed ID : 32873198
- PubMed Central 記事ID : PMC7542792