論文

査読有り
2008年

Effects of relaxin and IGF-I on capacitation, acrosome reaction, cholesterol efflux and utilization of labeled and unlabeled glucose in porcine spermatozoa

Reproductive Medicine and Biology
  • Abdul Gaffar Miah
  • ,
  • Ummay Salma
  • ,
  • Yuji Takagi
  • ,
  • Tetsuya Kohsaka
  • ,
  • Ko-Ichi Hamano
  • ,
  • Hirotada Tsujii

7
1
開始ページ
29
終了ページ
36
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.1447-0578.2007.00198.x
出版者・発行元
John Wiley and Sons Ltd

Aim: Relaxin and insulin-like growth factor (IGF)-I have pronounced effects on the male and female reproductive tracts. The aim of this study was to investigate the effects of relaxin and IGF-I on the motility, capacitation, acrosome reaction, cholesterol efflux and utilization of glucose in porcine spermatozoa. Methods: Swim-up separated spermatozoa that had been washed twice were incubated at 37°C for 1 or 4.h in modified Tyrode's albumin lactate pyruvate (mTALP) medium supplemented without (control) or with relaxin (20.ng/mL) or IGF-I (20.ng/mL) or both (10 + 10.ng/mL). Results: Progressive motility and the induction rate of capacitation and acrosome reaction were increased (P &lt
0.05) by relaxin and IGF-I alone or in combination, especially after 4.h of incubation. Relaxin alone or combined with IGF-I enhanced (P &lt
0.05) the cholesterol efflux after 4.h, whereas IGF-I alone did not show any significant effect on the cholesterol efflux compared with the control at any time point. The utilization rates of labeled and unlabeled glucose increased (P &lt
0.05) in spermatozoa incubated with relaxin or IGF-I alone or in combination compared with the control. Conclusion: Thus, supplementation of relaxin alone or combined with IGF-I into the medium possibly plays a beneficial role in porcine spermatozoal prefertilization events in vitro. Journal compilation © 2008 Japan Society for Reproductive Medicine.

リンク情報
DOI
https://doi.org/10.1111/j.1447-0578.2007.00198.x
ID情報
  • DOI : 10.1111/j.1447-0578.2007.00198.x
  • ISSN : 1447-0578
  • ISSN : 1445-5781
  • SCOPUS ID : 38949114880

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