2008年
Effects of relaxin and IGF-I on capacitation, acrosome reaction, cholesterol efflux and utilization of labeled and unlabeled glucose in porcine spermatozoa
Reproductive Medicine and Biology
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- 巻
- 7
- 号
- 1
- 開始ページ
- 29
- 終了ページ
- 36
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1447-0578.2007.00198.x
- 出版者・発行元
- John Wiley and Sons Ltd
Aim: Relaxin and insulin-like growth factor (IGF)-I have pronounced effects on the male and female reproductive tracts. The aim of this study was to investigate the effects of relaxin and IGF-I on the motility, capacitation, acrosome reaction, cholesterol efflux and utilization of glucose in porcine spermatozoa. Methods: Swim-up separated spermatozoa that had been washed twice were incubated at 37°C for 1 or 4.h in modified Tyrode's albumin lactate pyruvate (mTALP) medium supplemented without (control) or with relaxin (20.ng/mL) or IGF-I (20.ng/mL) or both (10 + 10.ng/mL). Results: Progressive motility and the induction rate of capacitation and acrosome reaction were increased (P <
0.05) by relaxin and IGF-I alone or in combination, especially after 4.h of incubation. Relaxin alone or combined with IGF-I enhanced (P <
0.05) the cholesterol efflux after 4.h, whereas IGF-I alone did not show any significant effect on the cholesterol efflux compared with the control at any time point. The utilization rates of labeled and unlabeled glucose increased (P <
0.05) in spermatozoa incubated with relaxin or IGF-I alone or in combination compared with the control. Conclusion: Thus, supplementation of relaxin alone or combined with IGF-I into the medium possibly plays a beneficial role in porcine spermatozoal prefertilization events in vitro. Journal compilation © 2008 Japan Society for Reproductive Medicine.
0.05) by relaxin and IGF-I alone or in combination, especially after 4.h of incubation. Relaxin alone or combined with IGF-I enhanced (P <
0.05) the cholesterol efflux after 4.h, whereas IGF-I alone did not show any significant effect on the cholesterol efflux compared with the control at any time point. The utilization rates of labeled and unlabeled glucose increased (P <
0.05) in spermatozoa incubated with relaxin or IGF-I alone or in combination compared with the control. Conclusion: Thus, supplementation of relaxin alone or combined with IGF-I into the medium possibly plays a beneficial role in porcine spermatozoal prefertilization events in vitro. Journal compilation © 2008 Japan Society for Reproductive Medicine.
- ID情報
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- DOI : 10.1111/j.1447-0578.2007.00198.x
- ISSN : 1447-0578
- ISSN : 1445-5781
- SCOPUS ID : 38949114880