MISC

2004年7月

Radical scavenging activity and oxidative modification of citrus dehydrin

PLANT PHYSIOLOGY AND BIOCHEMISTRY
  • M Hara
  • ,
  • M Fujinaga
  • ,
  • T Kuboi

42
7-8
開始ページ
657
終了ページ
662
記述言語
英語
掲載種別
DOI
10.1016/j.plaphy.2004.06.004
出版者・発行元
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

Dehydrins are ubiquitous proteins produced by plants in response to water stress. Their functions, however, are not fully understood. The overexpression of Citrus unshiu Marcov. dehydrin (CuCOR19) enhanced cold tolerance in transgenic plants by reducing lipid peroxidation promoted by cold stress, suggesting that the CuCOR19 protein directly scavenges radicals. In this paper, we report the radical scavenging activity and oxidative modification of CuCOR19. The hydroxyl radical generated by the Fe2+/H2O2 system and peroxyl radical generated from 2,2'-azobis (2-amidinopropane) (AAPH) were scavenged by CuCOR19, but hydrogen peroxide and superoxide were not. The scavenging activity for the hydroxyl radical and peroxyl radical of CuCOR19 was more potent than that of mannitol, and approximately equal to that of serum albumin. which is known as an antioxidative protein in mammals. CuCOR19 was degraded by the hydroxyl radical and peroxyl radical in a time- and dose-dependent manner. Mannitol and thiourea inhibited the degradation. Analysis of the amino acid composition of CuCOR19 indicated that glycine. histidine, and lysine, which are major residues in many dehydrins, were targeted by the hydroxyl radical. These results suggest that CuCOR19 is a radical scavenging protein, and may reduce oxidative damage induced by water stress in plants. (C) 2004 Elsevier SAS. All rights reserved.

Web of Science ® 被引用回数 : 105

リンク情報
DOI
https://doi.org/10.1016/j.plaphy.2004.06.004
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000223780300012&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.plaphy.2004.06.004
  • ISSN : 0981-9428
  • Web of Science ID : WOS:000223780300012

エクスポート
BibTeX RIS