論文

査読有り
2008年5月

Macrophage colony-stimulating factor is indispensable for repopulation and differentiation of Kupffer cells but not for splenic red pulp macrophages in osteopetrotic (op/op) mice after macrophage depletion

CELL AND TISSUE RESEARCH
  • Takashi Yamamoto
  • ,
  • Chikako Kaizu
  • ,
  • Takashi Kawasaki
  • ,
  • Go Hasegawa
  • ,
  • Hajime Umezu
  • ,
  • Riuko Ohashi
  • ,
  • Junko Sakurada
  • ,
  • Shuying Jiang
  • ,
  • Leonard Shultz
  • ,
  • Makoto Naito

332
2
開始ページ
245
終了ページ
256
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00441-008-0586-8
出版者・発行元
SPRINGER

We previously reported that macrophage colony-stimulating factor (M-CSF, CSF-1) played important roles in the process of the repopulation of Kupffer cells after their elimination by administration of liposome-entrapped dichloromethylene diphosphonate (lipo-MDP). In this study, we examined the repopulation of Kupffer cells and splenic red pulp macrophages in osteopetrotic (op/op) mice defective in the production of functional M-CSF and their littermate mice by using the lipo-MDP model. In untreated op/op mice, numbers of F4/80-positive Kupffer cells in the liver and F4/80-positive splenic red pulp macrophages were reduced. Repopulation of Kupffer cells and splenic macrophages was observed in littermate (op/+) mice liver by 14 days after depletion. However, in op/op mice, repopulation of Kupffer cells was not observed in Kupffer-cell-depleted op/op mice until 56 days after depletion, whereas splenic red pulp macrophages repopulated and recovered to the level of control op/op mice by 10 days after depletion. Single injection of M-CSF was effective for the induction of the repopulation of Kupffer cells, and daily administration of M-CSF induced remarkable repopulation and maturation of Kupffer cells and proliferation of macrophage precursor cells in the liver of Kupffer-cell-depleted op/op mice. These results suggest that Kupffer cells are completely M-CSF-dependent tissue macrophages, whereas splenic red pulp macrophages are composed of M-CSF-dependent macrophages and M-CSF-independent macrophages. This mouse model provides a useful tool for the study of effects of growth factor on Kupffer cell differentiation in vivo.

リンク情報
DOI
https://doi.org/10.1007/s00441-008-0586-8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18335245
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000254956500006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00441-008-0586-8
  • ISSN : 0302-766X
  • PubMed ID : 18335245
  • Web of Science ID : WOS:000254956500006

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