MISC

2013年10月

Ultrastructure of Cement Lines

JOURNAL OF HARD TISSUE BIOLOGY
  • Hiroyuki Okada
  • ,
  • Ryo Tamamura
  • ,
  • Takeshi Kanno
  • ,
  • Hiroshi Nakada
  • ,
  • Saori Yasuoka
  • ,
  • Kazumune Arikawa
  • ,
  • Takao Kato
  • ,
  • Takashi Kaneda
  • ,
  • John E. Davies

22
4
開始ページ
445
終了ページ
449
記述言語
英語
掲載種別
出版者・発行元
JOURNAL HARD TISSUE BIOLOGY

Bone remodeling is achieved through the resorptive activity of osteoclasts and the synthetic activity of osteoblast and it continues throughout life. Cement lines are formed by osteoblasts and are recognized between old and new bone. However, the detailed structure of cement lines has still not been clarified. The present study researched the ultrastructure of cement lines in a ferret femur using light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). On LM, many resorption tunnels were recognized in cortical bones. Some osteoclasts in Howship's lacunae were observed at the top of the resorption tunnel (cutting cone), and osteoblasts forming some layers were recognized behind osteoclasts (filling cone). The layer of osteoid was observed under osteoblasts, and it was slightly eosinophilic on the decalcified specimen. A very thin layer of cement lines was recognized between osteoid and old bone, and it was slightly basophilic on hematoxylin and eosin (HE). On the non-decalcified sections stained with toluidine blue (TB), cement lines showed anochromasia in the non-etched specimen, but stained with TB in the etched one. On TEM, cement lines were recognized between osteoid and old bone, and they were revealed as extracellular matrices of an afibrillar layer, with a thickness of about 2 mu m. On BSE analysis, cement lines of osteon were observed as 1- 2 mu m white lines. Conclusively, the present study indicated that cement lines are extracellular matrices of hypercalcified afibrillar layer having 2 mu m thickness.

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000326421000008&DestApp=WOS_CPL
ID情報
  • ISSN : 1341-7649
  • Web of Science ID : WOS:000326421000008

エクスポート
BibTeX RIS