論文

査読有り
2002年3月

Domain structure of chondroitin sulfate E octasaccharides binding to type V collagen

JOURNAL OF BIOLOGICAL CHEMISTRY
  • K Takagaki
  • ,
  • H Munakata
  • ,
  • Kakizaki, I
  • ,
  • M Iwafune
  • ,
  • T Itabashi
  • ,
  • M Endo

277
11
開始ページ
8882
終了ページ
8889
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M106479200
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

We demonstrated previously that chondroitin sulfate E (ChS-E) binds to type V collagen (Munakata, H., Takagaki, K., Majima, M., and Endo, M. (1999) Glycobiology 9, 1023-1027). In this study, we investigated the structure and binding of ChS-E oligosaccharides. Eleven oligosaccharides were isolated from ChS-E by gel filtration chromatography and anion-exchange high performance liquid chromatography after hydrolysis with testicular hyaluronidase. Separately, seven oligosaccharides were custom synthesized using the transglycosylation reaction of testicular hyaluronidase. Structural analysis was performed by enzymatic digestions in conjunction with high performance liquid chromatography and mass spectrometry. This library of IS oligosaccharides was used as a source of model molecules to clarify the structural requirements for binding to type V collagen. Binding was analyzed by a biosensor based on surface plasmon resonance. The results indicated that to bind to type V collagen the oligosaccharides must have the following carbohydrate structures: 1) octasaccharide or larger in size; 2) a continuous sequence of three GlcAbeta1-3GalNAc(4S,6S) units; 3) a GlcAbeta1-3GalNAc(4S,6S) unit, GIcA/3I-3GaINAc(4S) unit or GlcAbeta1-3GalNAc(6S) unit at the reducing terminal; 4) a GlcAbeta1-3GalNAc(4S,6S) unit at the nonreducing terminal. It is likely that these characteristic oligosaccharide sequences play key roles in cell adhesion and extracellular matrix assembly.

Web of Science ® 被引用回数 : 44

リンク情報
DOI
https://doi.org/10.1074/jbc.M106479200
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/11751896
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000174400600021&DestApp=WOS_CPL

エクスポート
BibTeX RIS