論文

査読有り
2000年4月

Enzymatic reconstruction of dermatan sulfate

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • K Takagaki
  • ,
  • H Munakata
  • ,
  • Kakizaki, I
  • ,
  • M Majima
  • ,
  • M Endo

270
2
開始ページ
588
終了ページ
593
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1006/bbrc.2000.2452
出版者・発行元
ACADEMIC PRESS INC

We investigated the enzymatic reconstruction of dermatan sulfate (DS) using the transglycosylation reaction of testicular hyaluronidase. First, in order to insert the IdoA-GalNAc disaccharide unit into chondroitin sulfate chains consisting of GlcA-GalNAc disaccharide units, desulfated DS as a donor and pyridylaminated (PA) chondroitin g-sulfate (Ch6S) hexasaccharide as an acceptor were subjected to a transglycosylation reaction using testicular hyaluronidase. The products were analyzed by HPLC, mass spectrometry, and enzymatic digestions, and the results indicated that one of the products was IdoA-GalNAc-(GlcA-GalNAc6S)(3)-PA. Next, when the resulting PACh6S (hexa-)desulfated DS (di-)octasaccharide was used as an acceptor and chondroitin as a new donor, a decasaccharide having a GlcA-GalNAc-IdoA-GalNAc-(GlcA-GalNAc6S)(3) sequence was reconstructed. Using suitable combinations of donors and accepters, it was possible to custom synthesize DS having any IdoA sequence as its uronic acid component. It is likely that application of this system would facilitate artificial reconstruction of variant DS having different specific functions. (C) 2000 Academic Press.

Web of Science ® 被引用回数 : 28

リンク情報
DOI
https://doi.org/10.1006/bbrc.2000.2452
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/10753668
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000086525200043&DestApp=WOS_CPL

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