論文

査読有り 国際誌
2019年10月18日

Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7.

Nature communications
  • Kyoko Hanawa-Suetsugu
  • Yuzuru Itoh
  • Maisarah Ab Fatah
  • Tamako Nishimura
  • Kazuhiro Takemura
  • Kohei Takeshita
  • Satoru Kubota
  • Naoyuki Miyazaki
  • Wan Nurul Izzati Wan Mohamad Noor
  • Takehiko Inaba
  • Nhung Thi Hong Nguyen
  • Sayaka Hamada-Nakahara
  • Kayoko Oono-Yakura
  • Masashi Tachikawa
  • Kenji Iwasaki
  • Daisuke Kohda
  • Masaki Yamamoto
  • Akio Kitao
  • Atsushi Shimada
  • Shiro Suetsugu
  • 全て表示

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開始ページ
4763
終了ページ
4763
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41467-019-12738-w

Phagocytosis is a cellular process for internalization of micron-sized large particles including pathogens. The Bin-Amphiphysin-Rvs167 (BAR) domain proteins, including the FCH-BAR (F-BAR) domain proteins, impose specific morphologies on lipid membranes. Most BAR domain proteins are thought to form membrane invaginations or protrusions by assembling into helical submicron-diameter filaments, such as on clathrin-coated pits, caveolae, and filopodia. However, the mechanism by which BAR domain proteins assemble into micron-scale phagocytic cups was unclear. Here, we show that the two-dimensional sheet-like assembly of Growth Arrest-Specific 7 (GAS7) plays a critical role in phagocytic cup formation in macrophages. GAS7 has the F-BAR domain that possesses unique hydrophilic loops for two-dimensional sheet formation on flat membranes. Super-resolution microscopy reveals the similar assemblies of GAS7 on phagocytic cups and liposomes. The mutations of the loops abolishes both the membrane localization of GAS7 and phagocytosis. Thus, the sheet-like assembly of GAS7 plays a significant role in phagocytosis.

リンク情報
DOI
https://doi.org/10.1038/s41467-019-12738-w
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31628328
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802115
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-85073511723&partnerID=MN8TOARS
URL
http://orcid.org/0000-0002-4612-0628
ID情報
  • DOI : 10.1038/s41467-019-12738-w
  • ORCIDのPut Code : 65062561
  • PubMed ID : 31628328
  • PubMed Central 記事ID : PMC6802115
  • SCOPUS ID : 85073511723

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