論文

査読有り
2013年10月

A Simple Assay for Measuring Catalase Activity: A Visual Approach

SCIENTIFIC REPORTS
  • Tadayuki Iwase
  • ,
  • Akiko Tajima
  • ,
  • Shinya Sugimoto
  • ,
  • Ken-ichi Okuda
  • ,
  • Ippei Hironaka
  • ,
  • Yuko Kamata
  • ,
  • Koji Takada
  • ,
  • Yoshimitsu Mizunoe

3
開始ページ
3081
終了ページ
43104
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep03081
出版者・発行元
NATURE PUBLISHING GROUP

In this study, an assay that combines the ease and simplicity of the qualitative approach for measuring catalase activity was developed. The assay reagents comprised only hydrogen peroxide and Triton X-100. The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized as foam, whose height was estimated. A calibration plot using the defined unit of catalase activity yielded the best linear fit over a range of 20-300 units (U) (y = 0.3794x - 2.0909, r(2) = 0.993). The assay precision and reproducibility at 100 U were 4.6% and 4.8%, respectively. The applicability of the assay for measuring the catalase activity of various samples was assessed using laboratory strains of Escherichia coli, catalase-deficient isogenic mutants, clinically isolated Shiga toxin-producing E. coli, and human cells. The assay generated reproducible results. In conclusion, this new assay can be used to measure the catalase activity of bacterial isolates and human cells.

リンク情報
DOI
https://doi.org/10.1038/srep03081
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000326332500002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/srep03081
  • ISSN : 2045-2322
  • Web of Science ID : WOS:000326332500002

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