2013年10月
A Simple Assay for Measuring Catalase Activity: A Visual Approach
SCIENTIFIC REPORTS
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- 巻
- 3
- 号
- 開始ページ
- 3081
- 終了ページ
- 43104
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep03081
- 出版者・発行元
- NATURE PUBLISHING GROUP
In this study, an assay that combines the ease and simplicity of the qualitative approach for measuring catalase activity was developed. The assay reagents comprised only hydrogen peroxide and Triton X-100. The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized as foam, whose height was estimated. A calibration plot using the defined unit of catalase activity yielded the best linear fit over a range of 20-300 units (U) (y = 0.3794x - 2.0909, r(2) = 0.993). The assay precision and reproducibility at 100 U were 4.6% and 4.8%, respectively. The applicability of the assay for measuring the catalase activity of various samples was assessed using laboratory strains of Escherichia coli, catalase-deficient isogenic mutants, clinically isolated Shiga toxin-producing E. coli, and human cells. The assay generated reproducible results. In conclusion, this new assay can be used to measure the catalase activity of bacterial isolates and human cells.
- リンク情報
- ID情報
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- DOI : 10.1038/srep03081
- ISSN : 2045-2322
- Web of Science ID : WOS:000326332500002