MISC

2008年4月

Alternaria brassicae produces a host-speciric protein toxin from germinating spores on host leaves

PHYTOPATHOLOGY
  • R. Y. Parada
  • ,
  • E. Sakuno
  • ,
  • N. Mori
  • ,
  • K. Oka
  • ,
  • M. Egusa
  • ,
  • M. Kodama
  • ,
  • H. Otani

98
4
開始ページ
458
終了ページ
463
記述言語
英語
掲載種別
DOI
10.1094/PHYTO-98-4-0458
出版者・発行元
AMER PHYTOPATHOLOGICAL SOC

Spore suspensions of Alternaria brassicae, the causal agent of gray leaf spot in Brassica plants, were incubated on the leaves of cabbage (B. oleracea) and spore germination fluid (SGF) was collected after 48 h. A high molecular weight (HMW) fraction (> 10 kDa) was separated from the SGF by ultrafiltration. In a detached leaf assay, the HMW fraction induced visible symptoms only on host leaves and the toxicity was lost by treatment with proteinase K or heat at 60 degrees C for 15 min, indicating the presence of host-specific protein toxin(s). A protein toxin in the HMW fraction was purified by several chromatography steps. The toxin induced water-soaked symptoms followed by chlorosis at concentrations of 0.5 to 1 mu g/ml on host leaves, but not on nonhost leaves even at 50 mu g/ml. The toxin also had infection-inducing activity when added to spore suspension of a nonpathogenic isolate of A. alternata, causing symptoms similar to the infection of A. brassicae only on host leaves. These results indicate that a new host-specific protein toxin named ABR-toxin is released from germinating spores of A. brassicae on host leaves. ABR-toxin migrated as a protein of 27.5 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The isoelectric point of ABR-toxin was estimated to be approximately 7.0 and 21 N-terminal amino acid residues were sequenced.

Web of Science ® 被引用回数 : 17

リンク情報
DOI
https://doi.org/10.1094/PHYTO-98-4-0458
CiNii Articles
http://ci.nii.ac.jp/naid/80019465496
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000254310500012&DestApp=WOS_CPL
ID情報
  • DOI : 10.1094/PHYTO-98-4-0458
  • ISSN : 0031-949X
  • eISSN : 1943-7684
  • CiNii Articles ID : 80019465496
  • Web of Science ID : WOS:000254310500012

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