Tadashi Suzuki - My portal

Profile Information

Affiliation: Chief Scientist, Cluster for Pioneering Research, RIKEN

Degree: Master of Sciences(The University of Tokyo)
Doctor of Sciences(The University of Tokyo)

J-GLOBAL ID: 200901004108662011
researchmap Member ID: 5000046497

Research Interests

Research Areas

Life sciences / Functional biochemistry /

Research History

Apr, 2018 - Present

Chief Scientist, Glycometabolic Biochemistry Laboratory, Cluster for Pioneering Research (CPR), RIKEN
Apr, 2017 - Mar, 2024

Principal Investigator, NGLY1 Deficiency Project, T-CiRA (joint research program by Takeda and the Center for iPS Cell Research and Application (CiRA), Kyoto University
Oct, 2007 - Mar, 2018

Team Leader, Glycometabolome Team, Systems Glycobiology Research Group, RIKEN
Jan, 2004 - Sep, 2007

Visiting Associate Professor, Graduate School of Medicine, Osaka University
Dec, 2001 - Mar, 2005

Researcher, PRESTO, Japan Science and Technology Agency
Feb, 2002 - Jan, 2004

Specially-appointed Assistant Professor, Undergraduate Program for Bioinformatics and Systems Biology, Graduate School of Science, University of Tokyo,
Jul, 2000 - Mar, 2002

Research Assistant Professor, State University of New York at Stony Brook
Apr, 2000 - Nov, 2001

Research Scientist, State University of New York at Stony Brook
Apr, 1998 - Mar, 2000

Oversea Research Fellow, Japan Society for the Promotion of Science (JSPS)
Jul, 1997 - Mar, 2000

Postdoctoral Research Fellow,, State University of New York at Stony Brook
Apr, 1996 - Mar, 1998

Predoctoral Research Fellow, Japan Society for the Promotion of Science (JSPS)

Education

Apr, 1990 - Mar, 1992

Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo

Awards

Aug, 2023

IGO Hakomori Award, International Glycoconjugate Organization
Oct, 2022

JB Reviewer Award, Japanese Biochemical Society
Nov, 2016

Glycobiology Significant Achievement Award, Society for Glycobiology
Oct, 2009

Samuro Kakiuchi Memorial Research Award for Young Scientists, Japanese Biochemical Society
Aug, 2008

Young Investigator Award, Japanese Society for Carbohydrate Research
Oct, 2005

Young Investigator Award, Japan Biochemical Society
May, 2001

Young Scientist Award, International Symposium on Protein Traffic, Glycosylation, and Human Health (Interlaken)
Nov, 1997

Genzyme Award, Society for Glycobiology
Sep, 1997

Young Scientist Travel Award, International Glycoconjugate Organization (GlycoXIV, Zuerich)
Jul, 1997

Travel Award for 17th IUBMB Meeting, Japanese Biochemical Society
1997

International Exchange Fellowship, Kato Memorial Bioscience Foundation
Feb, 1996

FCCA Award for Young Researcher, FCCA (Forum: Carbohydrate Coming of Age)

Major Papers

132

Sugar-mediated non-canonical ubiquitination impairs Nrf1/NFE2L1 activation

Yukiko Yoshida, Tsuyoshi Takahashi, Nozomi Ishii, Ichiro Matsuo, Satoshi Takahashi, Haruka Inoue, Akinori Endo, Hikaru Tsuchiya, Meari Okada, Chikara Ando …

Molecular Cell, 84(16) 3115-3127.e11, Aug, 2024
Intranasal oxytocin suppresses seizure-like behaviors in a mouse model of NGLY1 deficiency

Yukimasa Makita, Makoto Asahina, Reiko Fujinawa, Hiroshi Yukitake, Tadashi Suzuki

Communications Biology, 7(1) 460-460, Apr 22, 2024
Development of a fluorescence and quencher-based FRET assay for detection of endogenous peptide:N-glycanase/NGLY1 activity

Hiroto Hirayama, Yuriko Tachida, Reiko Fujinawa, Yuka Matsuda, Takefumi Murase, Yuji Nishiuchi, Tadashi Suzuki

Journal of Biological Chemistry, 300(4) 107121-107121, Apr, 2024
Rat hepatocytes secrete free oligosaccharides

Chengcheng Huang, Junichi Seino, Akinobu Honda, Haruhiko Fujihira, Di Wu, Kyohei Okahara, Shinobu Kitazume, Shuichi Nakaya, Ken Kitajima, Chihiro Sato …

Journal of Biological Chemistry, 300(3) 105712-105712, Mar, 2024
Hydrolytic activity of yeast oligosaccharyltransferase is enhanced when misfolded proteins accumulate in the endoplasmic reticulum

Sheng‐Tao Li, Hiroto Hirayama, Chengcheng Huang, Tsugiyo Matsuda, Ritsuko Oka, Takahiro Yamasaki, Daisuke Kohda, Tadashi Suzuki

The FEBS Journal, 291(5) 884-896, Mar, 2024
Loss of peptide:N-glycanase causes proteasome dysfunction mediated by a sugar-recognizing ubiquitin ligase.

Yukiko Yoshida, Makoto Asahina, Arisa Murakami, Junko Kawawaki, Meari Yoshida, Reiko Fujinawa, Kazuhiro Iwai, Ryuichi Tozawa, Noriyuki Matsuda, Keiji Tanaka …

Proceedings of the National Academy of Sciences of the United States of America, 118(27), Jul 6, 2021
Reversibility of motor dysfunction in the rat model of NGLY1 deficiency

Makoto Asahina, Reiko Fujinawa, Hiroto Hirayama, Ryuichi Tozawa, Yasushi Kajii, Tadashi Suzuki

Molecular Brain, 14(1), Jun 13, 2021
JF1/B6F1 Ngly1-/- mouse as an isogenic animal model of NGLY1 deficiency.

Makoto Asahina, Reiko Fujinawa, Haruhiko Fujihira, Yuki Masahara-Negishi, Tomohiro Andou, Ryuichi Tozawa, Tadashi Suzuki

Proceedings of the Japan Academy. Series B, Physical and biological sciences, 97(2) 89-102, 2021
Glycometabolic Regulation of the Biogenesis of Small Extracellular Vesicles

Yoichiro Harada, Kazuki Nakajima, Takehiro Suzuki, Tomoko Fukushige, Kiyotaka Kondo, Junichi Seino, Yuki Ohkawa, Tadashi Suzuki, Hiromasa Inoue, Takuro Kanekura …

Cell Reports, 33(2) 108261-108261, Oct, 2020
Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation

Antonio Galeone, Joshua M Adams, Shinya Matsuda, Maximiliano F Presa, Ashutosh Pandey, Seung Yeop Han, Yuriko Tachida, Hiroto Hirayama, Thomas Vaccari, Tadashi Suzuki …

eLife, 9, Jul 28, 2020
The ER-associated protease Ste24 prevents N-terminal signal peptide-independent translocation into the endoplasmic reticulum in Saccharomyces cerevisiae

Akira Hosomi, Kazuko Iida, Toshihiko Cho, Hidetoshi Iida, Masashi Kaneko, Tadashi Suzuki

Journal of Biological Chemistry, 295(30) 10406-10419, Jul, 2020
Ngly1 −/− rats develop neurodegenerative phenotypes and pathological abnormalities in their peripheral and central nervous systems

Makoto Asahina, Reiko Fujinawa, Sayuri Nakamura, Kotaro Yokoyama, Ryuichi Tozawa, Tadashi Suzuki

Human Molecular Genetics, 29(10) 1635-1647, Apr 7, 2020
Liver-specific deletion of Ngly1 causes abnormal nuclear morphology and lipid metabolism under food stress

Haruhiko Fujihira, Yuki Masahara-Negishi, Yoshihiro Akimoto, Hiroto Hirayama, Hyeon-Cheol Lee, Benjamin A. Story, William F. Mueller, Petra Jakob, Sandra Clauder-Münster, Lars M. Steinmetz …

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1866(3) 165588-165588, Mar, 2020
A bioactive mammalian disaccharide associated with autoimmunity activates STING-TBK1-dependent immune response

Charles S. Fermaintt, Kanae Sano, Zhida Liu, Nozomi Ishii, Junichi Seino, Nicole Dobbs, Tadashi Suzuki, Yang-Xin Fu, Mark A. Lehrman, Ichiro Matsuo …

Nature Communications, 10(1), May 30, 2019
Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast

Hirayama, H., Matsuda, T., Tsuchiya, Y., Oka, R., Seino, J., Huang, C., Nakajima, K., Noda, Y., Shichino, Y., Iwasaki, S. …

Journal of Biological Chemistry, 294(44) 15900-15911, 2019
DNA tags used to image sugar-bearing proteins on cells.

Tadashi Suzuki

Nature, Sep 1, 2018
N-glycanase NGLY1 regulates mitochondrial homeostasis and inflammation through NRF1

Kun Yang, Ryan Huang, Haruhiko Fujihira, Tadashi Suzuki, Nan Yan

Journal of Experimental Medicine, 215(10) 2600-2616, Aug 22, 2018
Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene

Fujihira, H., Masahara-Negishi, Y., Tamura, M., Huang, C., Harada, Y., Wakana, S., Takakura, D., Kawasaki, N., Taniguchi, N., Kondoh, G. …

PLoS Genetics, 13(4) e1006696, 2017
Inhibition of NGLY1 Inactivates the Transcription Factor Nrf1 and Potentiates Proteasome Inhibitor Cytotoxicity

Tomlin, F.M., Gerling-Driessen, U.I.M., Liu, Y.-C., Flynn, R.A., Vangala, J.R., Lentz, C.S., Clauder-Muenster, S., Jakob, P., Mueller, W.F., Ordo{\~n}ez-Rueda, D. …

ACS Central Science, 3(11) 1143-1155, 2017
Tissue-specific regulation of BMP signaling by Drosophila N-glycanase

Galeone, A., Han, S.Y., Huang, C., Hosomi, A., Suzuki, T., Jafar-Nejad, H.

eLife, 6, 2017
Fluorogenic probes reveal a role of GLUT4 N-glycosylation in intracellular trafficking

Hirayama, S., Hori, Y., Benedek, Z., Suzuki, T., Kikuchi, K.

Nature Chemical Biology, 12(10) 853-859, 2016
Non-lysosomal degradation of singly phosphorylated oligosaccharides initiated by the action of a cytosolic endo-β-N-acetylglucosaminidase

Harada, Y., Huang, C., Yamaki, S., Dohmae, N., Suzuki, T.

Journal of Biological Chemistry, 291(15) 8048-8058, 2016
Identification of PNGase-dependent ERAD substrates in Saccharomyces cerevisiae

Hosom, A., Fujita, M., Tomioka, A., Kaji, H., Suzuki, T.

Biochemical Journal, 473(19) 3001-3012, 2016
Generation and degradation of free asparagine-linked glycans

Harada, Y., Hirayama, H., Suzuki, T.

Cellular and Molecular Life Sciences, 72(13) 2509-2533, 2015
Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway

Ninagawa, S., Okada, T., Sumitomo, Y., Horimoto, S., Sugimoto, T., Ishikawa, T., Takeda, S., Yamamoto, T., Suzuki, T., Kamiya, Y. …

Journal of Cell Biology, 211(4) 775-784, 2015
Endo-β-n-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in NGLY1-defective cells

Suzuki, T., Huang, C., Harada, Y., Hosomi, A., Masahara-Negishi, Y., Seino, J., Fujihira, H., Funakoshi, Y., Suzuki, T., Dohmae, N.

Proceedings of the National Academy of Sciences of the United States of America, 112(5) 1398-1403, 2015
Metabolically programmed quality control system for dolichol-linked oligosaccharides

Harada, Y., Nakajima, K., Masahara-Negish, Y., Freeze, H.H., Angata, T., Taniguchi, N., Suzuki, T.

Proceedings of the National Academy of Sciences of the United States of America, 110(48) 19366-19371, 2013
Dual functions for cytosolic α-mannosidase (Man2C1) its down-regulation causes mitochondria-dependent apoptosis independently of its α-mannosidase activity

Wang, L., Suzuki, T.

Journal of Biological Chemistry, 288(17) 11887-11896, 2013
Eukaryotic oligosaccharyltransferase generates free oligosaccharides during N-glycosylation

Harada, Y., Buser, R., Ngwa, E.M., Hirayama, H., Aebi, M., Suzuki, T.

Journal of Biological Chemistry, 288(45) 32673-32684, 2013
Basal autophagy is required for the efficient catabolism of sialyloligosaccharides

Seino, J., Wang, L., Harada, Y., Huang, C., Ishii, K., Mizushima, N., Suzuki, T.

Journal of Biological Chemistry, 288(37) 26898-26907, 2013
Visualizing specific protein glycoforms by transmembrane fluorescence resonance energy transfer

Haga, Y., Ishii, K., Hibino, K., Sako, Y., Ito, Y., Taniguchi, N., Suzuki, T.

Nature Communications, 3, 2012
N-glycosylation is critical for the stability and intracellular trafficking of glucose transporter GLUT4

Haga, Y., Ishii, K., Suzuki, T.

Journal of Biological Chemistry, 286(36) 31320-31327, 2011
Free oligosaccharides to monitor glycoprotein endoplasmic reticulum-associated degradation in Saccharomyces cerevisiae

Hirayama, H., Seino, J., Kitajima, T., Jigami, Y., Suzuki, T.

Journal of Biological Chemistry, 285(16) 12390-12404, 2010
Identification of an Htm1 (EDEM)-dependent, Mns1-independent endoplasmic reticulum-associated degradation (ERAD) pathway in Saccharomyces cerevisiae: Application of a novel assay for glycoprotein ERAD

Hosomi, A., Tanabe, K., Hirayama, H., Kim, I., Rao, H., Suzuki, T.

Journal of Biological Chemistry, 285(32) 24324-24334, 2010
The Neurospora peptide:N-glycanase ortholog PNG1 is essential for cell polarity despite its lack of enzymatic activity

Maerz, S., Funakoshi, Y., Negishi, Y., Suzuki, T., Seiler, S.

Journal of Biological Chemistry, 285(4) 2326-2332, 2010
The Png1-Rad23 complex regulates glycoprotein turnover

Kim, I., Ahn, J., Liu, C., Tanabe, K., Apodaca, J., Suzuki, T., Rao, H.

Journal of Cell Biology, 172(2) 211-219, 2006
Man2C1, an α-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol

Suzuki, T., Hara, I., Nakano, M., Shigeta, M., Nakagawa, T., Kondo, A., Funakoshi, Y., Taniguchi, N.

Biochemical Journal, 400(1) 33-41, 2006
Site-specific labeling of cytoplasmic peptide:N-glycanase by N,N′-diacetylchitobiose-related compounds

Suzuki, T., Hara, I., Nakano, M., Zhao, G., Lennarz, W.J., Schindelin, H., Taniguchi, N., Totani, K., Matsuo, I., Ito, Y.

Journal of Biological Chemistry, 281(31) 22152-22160, 2006
Genetic, biochemical, and morphological evidence for the involvement of N-glycosylation in biosynthesis of the cell wall β1,6-glucan of Saccharomyces cerevisiae

Chavan, M., Suzuki, T., Rekowicz, M., Lennarz, W.

Proceedings of the National Academy of Sciences of the United States of America, 100(26) 15381-15386, 2003
Endo-β- N -acetylglucosaminidase, an enzyme involved in processing of free oligosaccharides in the cytosol

Tadashi Suzuki, Keiichi Yano, Seiji Sugimoto, Ken Kitajima, William J. Lennarz, Sadako Inoue, Yasuo Inoue, Yasufumi Emori

Proceedings of the National Academy of Sciences, 99(15) 9691-9696, Jul 11, 2002
Site-directed mutagenesis study of yeast peptide: N-glycanase. Insight into the reaction mechanism of deglycosylation

Katiyar, S., Suzuki, T., Balgobin, B.J., Lennarz, W.J.

Journal of Biological Chemistry, 277(15) 12953-12959, 2002
Identification of proteins that interact with mammalian peptide: N-glycanase and implicate this hydrolase in the proteasome-dependent pathway for protein degradation

H. Park, T. Suzuki, W. J. Lennarz

Proceedings of the National Academy of Sciences of the United States of America, 98(20) 11163-11168, Sep 25, 2001
Rad23 Provides a Link between the Png1 Deglycosylating Enzyme and the 26 S Proteasome in Yeast

Suzuki, T., Park, H., Kwofie, M.A., Lennarz, W.J.

Journal of Biological Chemistry, 276(24), 2001
PNG1, a yeast gene encoding a highly conserved peptide: N-glycanase

Suzuki, T., Park, H., Hollingsworth, N.M., Sternglanz, R., Lennarz, W.J.

Journal of Cell Biology, 149(5), 2000
Peptides glycosylated in the endoplasmic reticulum of yeast are subsequently deglycosylated by a soluble peptide: N-glycanase activity

Suzuki, T., Park, H., Kitajima, K., Lennarz, W.J.

Journal of Biological Chemistry, 273(34) 21526-21530, 1998
Complex, two-way traffic of molecules across the membrane of the endoplasmic reticulum

Suzuki, T., Yan, Q., Lennarz, W.J.

Journal of Biological Chemistry, 273(17) 10083-10086, 1998
Combined chemical and enzymatic synthesis of a C-glycopeptide and its inhibitory activity toward glycoamidases

Wang, L.-X., Tang, M., Suzuki, T., Kitajima, K., Inoue, Y., Inoue, S., Fan, J.-Q., Lee, Y.C.

Journal of the American Chemical Society, 119(46) 11137-11146, 1997
Site-specific de-N-glycosylation of diglycosylated ovalbumin in hen oviduct by endogenous peptide: N-glycanase as a quality control system for newly synthesized proteins

Suzuki, T., Kitajima, K., Emori, Y., Inoue, Y., Inoue, S.

Proceedings of the National Academy of Sciences of the United States of America, 94(12) 6244-6249, 1997
Carbohydrate-binding property of peptide:N-glycanase from mouse fibroblast L-929 cells as evaluated by inhibition and binding experiments using various oligosaccharides

Tadashi Suzuki, Ken Kitajima, Yasuo Inoue, Sadako Inoue

Journal of Biological Chemistry, 270(25) 15181-15186, Jun 23, 1995
Purification and enzymatic properties of peptide:N-glycanase from C3H mouse-derived L-929 fibroblast cells. Possible widespread occurrence of post- translational remodification of proteins by N-deglycosylation

Suzuki, T., Seko, A., Kitajima, K., Inoue, Y., Inoue, S.

Journal of Biological Chemistry, 269(26) 17611-17618, 1994

Major Misc.

Biological function of peptide: V-glycanase in hen oviduct

Suzuki, T., Kitajima, K., Emori, Y., Inoue, Y., Inoue, S.

FASEB Journal, 11(9), 1997

Books and Other Publications

Sugar chains : decoding the functions of glycans

Suzuki, Tadashi, 大坪, 和明, 谷口, 直之

Springer, 2015 (ISBN: 9784431553809)

Professional Memberships

Research Projects

Clarification of novel glycan catabolic pathways by free glycan analysis of budding yeast and its application to human rare genetic disorders

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2023

Tadashi Suzuki
糖タンパク質の品質管理における糖鎖機能の解明

ＪＳＴ戦略的創造研究推進制度（研究チーム型）（戦略的基礎研究推進事業：ＣＲＥＳＴ）, 2002 - 2007
Clarification of Function of Glycan Chains on Quality Control of Glycoproteins

JST Basic Research Programs (Core Research for Evolutional Science and Technology :CREST), 2002 - 2007
小胞体におけるタンパク質品質管理機構に関わるPNGaseの構造と機能

ＪＳＴ戦略的創造研究推進制度（個人研究型）（個人研究推進事業：さきがけ研究２１‐ＰＲＥＳＴＯ）, 2001 - 2004
Structure and Functions of PNGase Implicated in the ER Quality Control System

JST Basic Research Programs (Precursory Research for Embryonic Science and Technology :PRESTO), 2001 - 2004