論文

査読有り
2019年

Identification of Intermediates in Peroxidase Catalytic Cycle of a DNAzyme Possessing Heme

Bulletin of the Chemical Society of Japan
  • Shinomiya, Ryosuke
  • ,
  • Araki, Haruka
  • ,
  • Momotake, Atsuya
  • ,
  • Kotani, Hiroaki
  • ,
  • Kojima, Takahiko
  • ,
  • Yamamoto, Yasuhiko

92
10
開始ページ
1729
終了ページ
1736
記述言語
日本語
掲載種別
研究論文(学術雑誌)
DOI
10.1246/bcsj.20190157
出版者・発行元
公益社団法人 日本化学会

<p>Heme in the ferric state (heme(Fe3+)) binds to G-quadruplex DNAs to form stable complexes that exhibit enhanced peroxidase activities. The complexes are considered DNAzymes possessing heme as a prosthetic group (heme-DNAzymes), and have been extensively investigated as promising catalysts for a variety of applications. On ESR and stopped-flow measurements, an iron(IV)oxo porphyrin π-cation radical known as Compound I was detected in reaction mixtures of heme-DNAzymes and hydrogen peroxide. This finding not only resolved the long-standing issue of the mechanism underlying the enhancement of the peroxidase activity of heme(Fe3+) in the scaffold of a G-quadruplex DNA, but also provided new insights as to the design of novel heme-DNAzymes.</p>

リンク情報
DOI
https://doi.org/10.1246/bcsj.20190157
ID情報
  • DOI : 10.1246/bcsj.20190157
  • ISSN : 0009-2673

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