論文

2018年9月

Characterization of Catalytic Activities and Heme Coordination Structures of Heme-DNA Complexes Composed of Some Chemically Modified Hemes and an All Parallel-Stranded Tetrameric G-Quadruplex DNA Formed from d(TTAGGG)

Biochemistry
  • Shinomiya, Ryosuke
  • ,
  • Katahira, Yuya
  • ,
  • Araki, Haruka
  • ,
  • Shibata, Tomokazu
  • ,
  • Momotake, Atsuya
  • ,
  • Yanagisawa, Sachiko
  • ,
  • Ogura, Takashi
  • ,
  • Suzuki, Akihiro
  • ,
  • Neya, Saburo
  • ,
  • Yamamoto, Yasuhiko

57
41
開始ページ
5930
終了ページ
5937
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acs.biochem.8b00793
出版者・発行元
AMER CHEMICAL SOC

Heme binds selectively to the 3'-terminal G-quartet (G6 G-quartet) of an all parallel-stranded tetrameric G-quadruplex DNA, [d(TTAGGG)], to form a heme-DNA complex. Complexes between [d(TTAGGG)] and a series of chemically modified hemes possessing a heme Fe atom with a variety of electron densities were characterized in terms of their peroxidase activities to evaluate the effect of a change in the electron density of the heme Fe atom (ρ) on their activities. The peroxidase activity of a complex decreased with a decreasing ρ, supporting the idea that the activity of the complex is elicited through a reaction mechanism similar to that of a peroxidase. In the ferrous heme-DNA complex, carbon monoxide (CO) can bind to the heme Fe atom on the side of the heme opposite the G6 G-quartet, and a water molecule (HO) is coordinated to the Fe atom as another axial ligand, trans to the CO. The stretching frequencies of Fe-bound CO (ν) and the Fe-C bond (ν) of CO adducts of the heme-DNA complexes were determined to investigate the structural and electronic natures of the axial ligands coordinated to the heme Fe atom. Comparison of the ν and ν values of the heme-DNA complexes with those of

リンク情報
DOI
https://doi.org/10.1021/acs.biochem.8b00793
ID情報
  • DOI : 10.1021/acs.biochem.8b00793
  • ISSN : 1520-4995

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