論文

査読有り
2020年

Characterization of Structure and Catalytic Activity of a Complex between Heme and an All Parallel-stranded Tetrameric G-quadruplex Formed from DNA/RNA Chimera Sequence d(TTA)r(GGG)dT

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
  • Kosuke, Hayasaka
  • ,
  • Tomokazu, Shibata
  • ,
  • Aya, Sugahara
  • ,
  • Atsuya, Momotake
  • ,
  • Toru, Matsui
  • ,
  • Saburo, Neya
  • ,
  • Takumi, Ishizuka
  • ,
  • Yan, Xu
  • ,
  • Yasuhiko, Yamamoto

93
5
開始ページ
621
終了ページ
629
記述言語
日本語
掲載種別
研究論文(学術雑誌)
DOI
10.1246/bcsj.20200013
出版者・発行元
CHEMICAL SOC JAPAN

An all-parallel tetrameric G-quadruplex formed from a DNA/RNA chimera sequence, d(TTA)r(GGG)d(T) ([Chimera(7mer)](4)), and its interaction with heme have been characterized. [Chimera(7mer)](4) was found to be remarkably more stable compared with a G-quadruplex formed from its DNA counterpart d(TTAGGGT), ([DNA(7mer)](4)), despite the structural similarity between them, and the increased stability of [Chimera(7mer)](4) is likely to be due to the formation of interstrand hydrogen bonds between the rG5 2'-OH and rG6 amino NH2 groups. Heme bound selectively to the 3'-terminal G-quartet (rG6 G-quartet) in the rG6dT7 step of [Chimera(7mer)](4) did not exhibit the heme orientational disorder resulting from the formation of two isomers possessing heme orientations differing by 180 degrees rotation about the pseudo-C-2 axis, with respect to the interacting G-quartet, although it is a general feature of heme-DNA complexes. In a complex between heme and [Chimera(7mer)](4), the predominance of one isomer in the heme orientational disorder over the other one is possibly due to contacts between the rG6 2'-OH group of the G-quartet and heme side chain ones. Peroxidase activity of heme bound to a G

リンク情報
DOI
https://doi.org/10.1246/bcsj.20200013
ID情報
  • DOI : 10.1246/bcsj.20200013
  • ISSN : 0009-2673

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