論文

査読有り
2020年4月

Stepwise binding of a cationic phthalocyanine derivative to an all parallel-stranded tetrameric G-quadruplex DNA

Journal of inorganic biochemistry
  • Uchiyama, Mami
  • ,
  • Okamoto, China
  • ,
  • Momotake, Atsuya
  • ,
  • Ikeue, Takahisa
  • ,
  • Yamamoto, Yasuhiko

213
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jinorgbio.2020.111270

Interaction between an Ga(III) phthalocyanine (Pc) derivative bearing eight N-methylpyridinium groups at peripheral β-positions (2,3,6,7,10,11,14,15-octakis-[N-methyl-(4-methylpyridinium-3-yloxy)phthalocyaninato] chloro gallium(III) iodide (GaPc)) and an all parallel-stranded tetrameric G-quadruplex formed from a heptanucleotide d(TTAGGGT) ([d(TTAGGGT)]) has been investigated to elucidate the molecular recognition of G-quadruplex DNA by the Pc derivative, which provides a useful insight as to the design of G-quadruplex ligands suitable for various in vitro and in vivo applications. We found that GaPc binds to the A3G4 and G6T7 steps of [d(TTAGGGT)], with binding constants of (21 ± 2) × 10 and (0.09 ± 0.06) × 10 M, respectively, to form a 2:1 complex. Obviously, upon the binding of GaPc to each of the sites, the π-π stacking and electrostatic interactions of the Pc moiety and positively-charged side chains of GaPc with a G-quartet and the negatively-charged phosphate groups in nearby phosphodiester bonds of the DNA, respectively, are major driving forces for the complexation. Considering the similarity in the local structural environment between the A3G4 and G6T7 ste

リンク情報
DOI
https://doi.org/10.1016/j.jinorgbio.2020.111270
ID情報
  • DOI : 10.1016/j.jinorgbio.2020.111270
  • ISSN : 1873-3344

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