論文

査読有り
2021年3月

Structural and functional characterization of complexes between heme and dimeric parallel G-quadruplex DNAs

Journal of inorganic biochemistry
  • Okamoto, China
  • ,
  • Momotake, Atsuya
  • ,
  • Yamamoto, Yasuhiko

216
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jinorgbio.2020.111336

Heme has been receiving considerable interest as a prosthetic group of ribozymes and deoxyribozymes, because heme-bound nucleic acids exhibit peroxidase-like catalytic activities (Travascio, P., Li, Y., and Sen, D. (1998) Chem. Biol, 5, 505-517). The interaction of heme with dimeric G-quadruplexes formed from d(TAGGGTTAGGGT) and d(TAGGGTTAGGGA) has been characterized to gain a deeper understanding of the molecular recognition of G-quadruplex DNAs by heme. We found that heme binds selectively to the 3'-terminal G-quartet of a dimeric parallel G-quadruplex of d(TAGGGTTAGGGT), whereas binding of heme to a dimeric antiparallel G-quadruplex of d(TAGGGTTAGGGA) does not occur, suggesting that an orderly arrangement of the constituent guanine deoxyribose rings, with respect to the G-quartet plane, is crucial for the binding of heme to the DNA. The preferential binding of heme to the 3'-terminal G-quartet of parallel G-quadruplex DNAs allowed a systematic modification of the heme environment in the complex through the DNA sequence. The activity of the complexes was found to increase with increasing number of adenine bases adjacent to the heme in the complexes, possibly due to improvement of

リンク情報
DOI
https://doi.org/10.1016/j.jinorgbio.2020.111336
ID情報
  • DOI : 10.1016/j.jinorgbio.2020.111336
  • ISSN : 1873-3344

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