論文

査読有り
2020年1月21日

Chromosome segregation error during early cleavage in mouse pre-implantation embryo does not necessarily cause developmental failure after blastocyst stage

Scientific Reports
  • Daisuke Mashiko
  • ,
  • Zenki Ikeda
  • ,
  • Tatsuma Yao
  • ,
  • Mikiko Tokoro
  • ,
  • Noritaka Fukunaga
  • ,
  • Yoshimasa Asada
  • ,
  • Kazuo Yamagata

10
1
開始ページ
854
終了ページ
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-020-57817-x
出版者・発行元
Springer Science and Business Media LLC

<title>Abstract</title>In the pre-implantation embryo, aneuploidy resulting from chromosome segregation error is considered responsible for pregnancy loss. However, only a few studies have examined the relationship between chromosome segregation errors during early cleavage and development. Here, we evaluated this relationship by live-cell imaging using the histone H2B-mCherry probe and subsequent single blastocyst transfer using mouse embryos obtained by <italic>in vitro</italic> fertilization. We showed that some embryos exhibiting early chromosomal segregation error and formation of micronuclei retained their developmental potential; however, the error affected the blastocyst/arrest ratio. Further, single-cell sequencing after live-cell imaging revealed that all embryos exhibiting micronuclei formation during 1st mitosis showed aneuploidy at the 2-cell stage. These results suggest that early chromosome segregation error causing micronuclei formation affects ploidy and development to blastocyst but does not necessarily cause developmental failure after the blastocyst stage. Our result suggests the importance of the selection of embryos that have reached blastocysts.

リンク情報
DOI
https://doi.org/10.1038/s41598-020-57817-x
URL
http://www.nature.com/articles/s41598-020-57817-x.pdf
URL
http://www.nature.com/articles/s41598-020-57817-x
ID情報
  • DOI : 10.1038/s41598-020-57817-x
  • eISSN : 2045-2322

エクスポート
BibTeX RIS