MISC

1996年3月

Interleukin-1 alpha activates all NF-kappa B-like factor in osteoclast-like cells

JOURNAL OF BIOLOGICAL CHEMISTRY
  • E Jimi
  • ,
  • T Ikebe
  • ,
  • N Takahashi
  • ,
  • M Hirata
  • ,
  • T Suda
  • ,
  • T Koga

271
9
開始ページ
4605
終了ページ
4608
記述言語
英語
掲載種別
DOI
10.1074/jbc.271.9.4605
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

We investigated the NF-kappa B transcription factor in osteoclast-like cells. Osteoclast-like cells were differentiated from mouse bone marrow cells in co-culture with mouse calvaria-derived primary osteoblasts in the presence of 1 alpha,25-dihydroxyvitamin D-3 and prostaglandin E(2) in collagen gel-coated dishes. We enriched osteoclastlike cells from the co-cultures by Pronase treatment. When the enriched osteoclast-like cells were treated with phorbol 12-myristate 13-acetate, interleukin-1 (IL-1), calcitonin, or macrophage colony-stimulating factor, only IL-1 activated an NF-kappa B-like factor, which specifically bound to a kappa B motif DNA sequence, as detected by an electrophoretic mobility shift assay. IL-1 also activated NF-kappa B induction in osteoblasts. However, the NF-kappa B-like factor induced by IL-1-stimulated osteoclast-like cells is of smaller molecular size than the factor in osteoblasts, as shown by an electrophoretic mobility shift assay. The NF-kappa B activity of osteoclast-like cells was recognized completely by antibodies against the p50 subunit, and only partially by antibodies against the p65 subunit of NF-kappa B. Antibodies against c-Rel, Rel B, and p52 did not recognize the NF-kappa B-like factor. These results suggest that IL-1 activates an NF-kappa B-like factor in osteoclast-like cells, which contains p50 and p65-related proteins.

Web of Science ® 被引用回数 : 74

リンク情報
DOI
https://doi.org/10.1074/jbc.271.9.4605
CiNii Articles
http://ci.nii.ac.jp/naid/30016342704
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/8617720
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1996TX69700010&DestApp=WOS_CPL