2011年12月
Identification and Characterization of a Mycobacterial (2R,3R)-2,3-Butanediol Dehydrogenase
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 75
- 号
- 12
- 開始ページ
- 2384
- 終了ページ
- 2389
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.110607
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Bacterial strain B-009, capable of using racemic 1,2-propanediol (PD), was identified as a rapid-growing member of the genus Mycobacterium. The strain is phylogenetically related to M. gilvum, but has slightly different physiological characteristics. An NAD(+)-dependent enantioselective alcohol dehydrogenase, which acts on R-PD, was purified from the strain. The enzyme was a homodimer of a peptide coded by a 1047-bp gene (mbd1). A highly conserved sequence for medium-chain dehydrogenase/reductases with a preference for secondary alcohols was found in the gene. Hydroxyacetone was produced from R-PD by an enzymatic reaction, indicating that position 2 of the substrate was oxidized. The enzyme activity was highest for (2R,3R)-2,3-butanediol (R,R-BD), enabling the enzyme to be identified as (2R,3R)-2,3-butanediol dehydrogenase (R,R-BD-DH). A homology search revealed M. gilvum, M. vanbaalenii, and M. semegmatis to have ORFs similar to mbd1, suggesting the widespread distribution of genes encoding R,R-BD-DH among mycobacterial strains.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.110607
- ISSN : 0916-8451
- eISSN : 1347-6947
- Web of Science ID : WOS:000298774700020