横川 隆志

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アバター
研究者氏名
横川 隆志
 
ヨコガワ タカシ
所属
岐阜大学
部署
工学部 化学・生命工学科 生命化学コース
職名
教授
学位
博士(理学)(東京工業大学)
その他の所属
研究推進・社会連携機構生命の鎖統合研究センターつくる領域連合創薬医療情報研究科工学研究科

プロフィール

非天然アミノ酸を含むタンパク質の合成/天然のタンパク質には見られないアミノ酸を含んだ高付加価値のタンパク質を合成する。

研究分野

 

経歴

 
2013年
   
 
岐阜大学 工学部 教授
 
 
   
 
岐阜大学 工学部 生命工学科 助教授
 
1995年12月
   
 
岐阜大学 講師
 
2007年4月
   
 
岐阜大学 准教授
 

学歴

 
1983年4月
 - 
1987年3月
東京大学工学部工業化学科  
 
1987年4月
 - 
1989年3月
東京大学大学院工学系研究科合成化学専攻修士課程  
 
1989年4月
 - 
1992年3月
東京工業大学大学院総合理工学研究科生命化学専攻博士課程  
 

受賞

 
1999年
日本生化学会JB論文賞
 

論文

 
Shinji Yamashita, Naoya Shibata, Akiyoshi Boku-Ikeda, Erika Abe, Ayumi Inayama, Takashi Yamaguchi, Ayano Higuma, Kaoru Inagaki, Tomoyo Tsuyuzaki, Satoshi Iwamoto, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Kazal Boron Biswas, A. H. M. Nurun Nabi, Tsutomu Nakagawa, Fumiaki Suzuki, Akio Ebihara
BMC Biotechnol   16 33   2016年4月   [査読有り]
Takuya Kawamura, Akira Hirata, Satoshi Ohno, Yuichiro Nomura, Tomoko Nagano, Nobukazu Nameki, Takashi Yokogawa, Hiroyuki Hori
Nucleic Acids Res   44(4) 1894-1908   2016年2月   [査読有り]
Yuichiro Nomura, Satoshi Ohno, Kazuya Nishikawa, Takashi Yokogawa
Genes to Cells   21 41-52   2016年1月   [査読有り]
© 2016 the Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd. In many archaeal tRNAs, archaeosine is found at position 15. During archaeosine biosynthesis, archaeal tRNA-guanine transglycosylase (ArcTGT) first replaces the gu...
Ai Kazayama, Ryota Yamagami, Takashi Yokogawa, Hiroyuki Hori
Journal of Biochemistry   157 411-418   2015年   [査読有り]
© 2015 The Authors 2015. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved. The solid-phase DNA probe method, in which a target transfer RNA (tRNA) is hybridized with a complementary DNA oligom...
Natsumi Inada, Kosuke Nakamoto, Takashi Yokogawa, Yoshihito Ueno, Yoshihito Ueno
European Journal of Medicinal Chemistry   103 460-472   2015年   [査読有り]
© 2015 Elsevier Masson SAS. All rights reserved. In this study, we aimed to create small interfering RNAs (siRNAs) with increased silencing activities and nuclease resistance properties. Therefore, we designed and synthesized five types of siRNA c...
Ikeda-Boku Akiyoshi, Kondo Keisuke, Ohno Satoshi, Yoshida Erika, Yokogawa Takashi, Hayashi Nobuhiro, Nishikawa Kazuya
Journal of biochemistry   154(2) 159-165   2013年8月   [査読有り]
We developed a novel method for capturing proteins that interact with a target protein. This method utilizes a protein containing a site-specifically incorporated 3-azidotyrosine (N3-Y) and FG beads for immobilization of the protein via an azido g...
Yuichiro Nomura, Yumiko Onda, Satoshi Ohno, Hiroki Taniguchi, Kaori Ando, Natsuhisa Oka, Kazuya Nishikawa, Takashi Yokogawa
Protein expression and purification   88(1) 13-19   2013年3月   [査読有り]
Many archaeal tRNAs have archaeosine (G(+)) at position 15 in the D-loop and this is thought to strengthen the tertiary interaction with C48 in the V-loop. In the first step of G(+) biosynthesis, archaeosinetRNA-guanine transglycosylase (ArcTGT)(1...
Ikeda-Boku Akiyoshi, Ohno Satoshi, Hibino Yuuka, Yokogawa Takashi, Hayashi Nobuhiro, Nishikawa Kazuya
Journal of biochemistry   153(3) 317-326   2013年3月   [査読有り]
We developed an efficient method for introduction of 3-azidotyrosine (N(3)-Y) into proteins in Escherichia coli cells. We constructed a plasmid that is adaptable for the constitutive expression of both Methanosarcina acetivorans tyrosyl-tRNA synth...
Hiroyuki Yamamoto, Rina Okada, Kazuaki Iguchi, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Keiko Unno, Minoru Hoshino, Atsushi Takeda
Biochemical and Biophysical Research Communications   430 999-1004   2013年   [査読有り]
Progalanin is released from the small cell lung carcinoma line SBC-3A and converted to its active form by plasmin. To elucidate the role of progalanin activation in the extracellular compartment, matrix metalloproteinase (MMP) activity was studied...
Shige H. Yoshimura, Shige H. Yoshimura, Shahbaz Khan, Shahbaz Khan, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Takamitsu Hosoya, Hiroyuki Maruyama, Yoshikazu Nakayama, Yoshikazu Nakayama, Kunio Takeyasu, Kunio Takeyasu
Bioconjugate Chemistry   23 1488-1493   2012年   [査読有り]
Establishing a nanobiohybrid device largely relies on the availability of various bioconjugation procedures which allow coupling of biomolecules and inorganic materials. Especially, site-specific coupling of a protein to nanomaterials is highly us...
Hiroyuki Yamamoto, Kazuaki Iguchi, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Minoru Hoshino
Protein and Peptide Letters   18(10) 1058-1064   2011年10月   [査読有り]
Galanin is a neuropeptide that is widely distributed in the central and peripheral nervous systems. Some small cell lung carcinoma (SCLC) cell lines such as SBC-3A release only the high-molecular-mass form, with lower molecular mass forms being un...
Fumie Iraha, Kenji Oki, Takatsugu Kobayashi, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Shigeyuki Yokoyama, Shigeyuki Yokoyama, Kensaku Sakamoto
Nucleic Acids Res   38(11) 3682-3691   2010年6月   [査読有り]
Non-natural amino acids have been genetically encoded in living cells, using aminoacyl-tRNA synthetase-tRNA pairs orthogonal to the host translation system. In the present study, we engineered Escherichia coli cells with a translation system ortho...
Takashi Yokogawa, Yusuke Kitamura, Daigo Nakamura, Satoshi Ohno, Kazuya Nishikawa
Nucleic Acids Res   38(6) e89   2010年4月   [査読有り]
We found that both tetramethylammonium chloride (TMA-Cl) and tetra-ethylammonium chloride (TEA-Cl), which are used as monovalent cations for northern hybridization, drastically destabilized the tertiary structures of tRNAs and enhanced the formati...
Tsutomu Suzuki, Yoshiho Ikeuchi, Satoshi Kimura, Tomoyuki Numata, Tomoyuki Numata, Daigo Nakamura, Takashi Yokogawa, Toshihiko Ogata, Takeshi Wada, Takeo Suzuki
Nat Chem Biol   6(4) 277-282   2010年2月   [査読有り]
A modified base at the first (wobble) positionof some tRNA anticodons is critical for deciphering the genetic code. In eukaryotes and eubacteria, AUA codons are decoded by tRNAsIle with modified bases pseudouridine (and/or inosine) and lysidine, r...
Chie Tomikawa, Takashi Yokogawa, Tamotsu Kanai, Hiroyuki Hori, Hiroyuki Hori, Hiroyuki Hori
Nucleic Acids Res   38(3) 942-957   2010年1月   [査読有り]
N(7)-methylguanine at position 46 (m(7)G46) in tRNA is produced by tRNA (m(7)G46) methyltransferase (TrmB). To clarify the roleof this modification, we made a trmB gene disruptant (DeltatrmB) of Thermus thermophilus, an extreme thermophilic eubact...
Takako Awai, Satoshi Kimura, Chie Tomikawa, Anna Ochi, Ihsanawati, Yoshitaka Bessho, Yoshitaka Bessho, Shigeyuki Yokoyama, Shigeyuki Yokoyama, Shigeyuki Yokoyama, Satoshi Ohno, Kazuya Nishikawa, Takashi Yokogawa, Tsutomu Suzuki, Hiroyuki Hori, Hiroyuki Hori, Hiroyuki Hori
J Biol Chem   284(31) 20467-20478   2009年7月   [査読有り]
Transfer RNA (N2,N2-guanine)-dimethyltransferase (Trm1) catalyzes N2,N2-dimethylguanine formation at position 26 (m(2)(2)G26) in tRNA. In the reaction, N2-guanine atposition 26 (m(2)G26) is generated as an intermediate. The trm1 genes are found on...
Chie Takemoto, Linda L. Spremulli, Lisa A. Benkowski, Takuya Ueda, Takuya Ueda, Takashi Yokogawa, Kimitsuna Watanabe, Kimitsuna Watanabe, Kimitsuna Watanabe
Nucleic Acids Res   37(5) 1616-1627   2009年4月   [査読有り]
Mitochondrial (mt) tRNA(Met) has the unusual modified nucleotide 5-formylcytidine (f(5)C) in the firstposition of the anticodon. This tRNA must translate both AUG and AUA as methionine. By constructingan in vitro translation system from bovine liv...
Masaru Tsunoda, Yoshio Kusakabe, Nobutada Tanaka, Satoshi Ohno, Masashi Nakamura, Toshiya Senda, Tomohisa Moriguchi, Norio Asai, Mitsuo Sekine, Takashi Yokogawa, Kazuya Nishikawa, Kazuo T. Nakamura
Nucleic Acids Res   35(13) 4289-4300   2007年5月   [査読有り]
The specific aminoacylation of tRNA by tyrosyl-tRNA synthetases (TyrRSs) relies on the identity determinants in the cognate tRNA(Tyr)s. We have determined the crystal structure of Saccharomyces cerevisiae TyrRS (SceTyrRS) complexed with a Tyr-AMP ...
Hideaki Shimizu, Shin ichi Yokobori, Takatoshi Ohkuri, Takashi Yokogawa, Kazuya Nishikawa, Akihiko Yamagishi
J MolBiol   369(4) 1060-1069   2007年4月   [査読有り]
Based on phylogenetic analysis of 16 S and 18 S rRNAs, the common ancestor of all organisms (Commonote) wasproposed to be hyperthermophilic. We have previously tested this hypothesis using enzymeswith ancestral residues that are inferred by molecu...
Masato Abe, Satoshi Ohno, Takashi Yokogawa, Takeshi Nakanishi, Fumio Arisaka, Takamitsu Hosoya, Toshiyuki Hiramatsu, Masaaki Suzuki, Tomio Ogasawara, Tatsuya Sawasaki, Tatsuya Sawasaki, Tatsuya Sawasaki, Kazuya Nishikawa, Masaya Kitamura, Hiroyuki Hori, Hiroyuki Hori, Hiroyuki Hori, Yaeta Endo, Yaeta Endo, Yaeta Endo
Proteins   67(3) 643-652   2007年3月   [査読有り]
A cell-free protein synthesis system is a powerful tool with which unnatural amino acids can be introduced into polypeptide chains. Here, the authors describe unnatural amino acid probing in a wheat germ cell-free translation system as a method fo...
Satoshi Ohno, Megumi Matsui, Takashi Yokogawa, Masashi Nakamura, Takamitsu Hosoya, Takamitsu Hosoya, Toshiyuki Hiramatsu, Toshiyuki Hiramatsu, Masaaki Suzuki, Nobuhiro Hayashi, Kazuya Nishikawa
J Biochem   141(3) 335-343   2007年1月   [査読有り]
An efficient method for site-selective modification of proteins using an unnatural amino acid, 3-azidotyrosine has beendeveloped. This method utilizes the yeast amber suppressor tRNA(Tyr)/mutated tyrosyl-tRNA synthetase pair as a carrier of 3-azid...
Jun-Ichi Fukunaga, Satoshi Ohno, Kazuya Nishikawa, Takashi Yokogawa
Nucleic Acids Res   34(10) 3181-3188   2006年5月   [査読有り]
Although the yeast amber suppressor tRNA(Tyr) is a good candidate for a carrier of unnatural amino acids into proteins, slight misacylation with lysine was found to occur in an Escherichia coli protein synthesis system. Although it was possible to...
Jun-Ichi Fukunaga, Takashi Yokogawa, Satoshi Ohno, Kazuya Nishikawa
Journal of Biochemistry   139 689-696   2006年4月
Through an exhaustive search for Escherichia coli aminoacyl-tRNA synthetase(s) responsible for the misacylation of yeast suppressor tRNA Tyr, E. coli lysyl-tRNA synthetase was found to have a weak activity to aminoacylate yeast amber suppressor tR...
Yoshio Kusakabe, Satoshi Ohno, Nobutada Tanaka, Masashi Nakamura, Masaru Tsunoda, Tomohisa Moriguchi, Norio Asai, Mitsuo Sekine, Takashi Yokogawa, Kazuya Nishikawa, Kazuo T. Nakamura
Protein Pept Lett.   13(4) 417-419   2006年4月   [査読有り]
Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNATyr. The crystals belong to a tetragonal space group P41212 with cell dimensions of a= b = 63.85 A°, and c = 330.3 A°. Th...
Jun-Ichi Fukunaga, Masaki Gouda, Koji Umeda, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa
J Biochem (Tokyo)   139(1) 123-127   2006年1月   [査読有り]
Because T7 RNA polymerase has a strong preference for particular sequences to initiate transcription, some RNAs having pyrimidine-rich sequences at their 5'-end (yeast tRNA(Tyr), for example) are hardly transcribed by this enzyme. To circumvent th...
Zemfira N. Karamysheva, Zemfira N. Karamysheva, Zemfira N. Karamysheva, Andrey L. Karamyshev, Andrey L. Karamyshev, Koichi Ito, Takashi Yokogawa, Kazuya Nishikawa, Yoshikazu Nakamura, Senya Matsufuji
Nucleic Acids Res   31(20) 5949-5956   2003年10月   [査読有り]
Translation termination in eukaryotes is mediated by the release factors eRF1 and eRF3, but mechanisms of the interplay between these factors are not fully understood, due partly to the difficulty of measuring termination on eukaryotic mRNAs. Here...
Masaki Gouda, Takashi Yokogawa, Haruichi Asahara, Kazuya Nishikawa
FEBS Lett   518(1-3) 139-143   2002年5月   [査読有り]
Class I aminoacyl-tRNA synthetases have been thought to be single polypeptide enzymes. However, the complete genome sequence of a hyper thermophile Aquifex aeolicus suggests that the gene for leucyl-tRNA synthetases (LeuRS) is probably split into ...
Ichiro Hirao, Takashi Ohtsuki, Tsuyoshi Fujiwara, Tsuneo Mitsui, Tomoko Yokogawa, Taeko Okuni, Hiroshi Nakayama, Koji Takio, Takashi Yabuki, Takanori Kigawa, Koichiro Kodama, Takashi Yokogawa, Kazuya Nishikawa, Shigeyuki Yokoyama
Nat Biotechnol   20(2) 177-182   2002年2月   [査読有り]
An unnatural base pair of 2-amino-6-(2-thienyl)purine (denoted by s) and pyridin-2-one (denoted by y) was developed to expand the genetic code. The ribonucleoside triphosphate of y was site-specifically incorporated into RNA, opposite s in a templ...
Masaki Gouda, Takashi Yokogawa, Kazuya Nishikawa
Biochemical and Biophysical Research Communications   297 950-955   2002年   [査読有り]
The active form of the leucyl-tRNA synthetase from an extreme thermophile Aquifex aeolicus has a heterodimeric (α/β type) quaternary structure that is unique among class I aminoacyl-tRNA synthetases. In an attempt to clarify the individual roles o...
Takao Hanada, Tsutomu Suzuki, Tsutomu Suzuki, Takashi Yokogawa, Chie Takemoto-Hori, Mathias Sprinzl, Kimitsuna Watanabe, Kimitsuna Watanabe
Genes Cells   6(12) 1019-1030   2001年12月   [査読有り]
BACKGROUND: Metazoan mitochondrial (mt) tRNAs are structurally quitedifferent from the canonical cloverleaf secondary structure. The mammalian mt tRNASerGCU for AGY codons (Y = C or U) lacks the entire D arm, whereas tRNASerUGA for UCN codons (N =...
Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa
J Biochem (Tokyo)   130(3) 417-423   2001年9月   [査読有り]
In an attempt to generate mutant aminoacyl-tRNA synthetases capable of charging non-canonical amino acids, a series of yeast tyrosyl-tRNA synthetase (TyrRS) mutants was constructed by site-specific mutagenesis of putative active site residues, whi...
Yoshihiro Shimizu, Akio Inoue, Yukihide Tomari, Tsutomu Suzuki, Takashi Yokogawa, Kazuya Nishikawa, Takuya Ueda
Nat Biotechnol   19(8) 751-755   2001年8月   [査読有り]
We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system wasable to produce protein at a rate of about 160 microg/ml/h in a batch mode without the need for any supple...
Takashi Yokogawa, Nobukazu Shimada, Nono Takeuchi, Lisa Benkowski, Tsutomu Suzuki, Akira Omori, Takuya Ueda, Kazuya Nishikawa, Linda L. Spremulli, Kimitsuna Watanabe
J Biol Chem   275(26) 19913-19920   2000年6月   [査読有り]
Animal mitochondrial protein synthesis systems contain two serine tRNAs (tRNAs(Ser)) corresponding to the codons AGY and UCN, each possessing an unusual secondary structure; the former lacks the entire D arm, and the latter has a slightly differen...
Satoshi Ohno, Takashi Yokogawa, Isao Fujii, Haruichi Asahara, Hachiro Inokuchi, Kazuya Nishikawa
J Biochem (Tokyo)   124(6) 1065-1068   1998年12月   [査読有り]
An efficient system was developed for the co-expression of a yeast tRNATyr/tyrosyl-tRNA synthetase (TyrRS) pair in Escherichia coli. Analysis of suppression patterns using several sets of E. coli and lambda phage mutants indicated that the express...
Ikuko Hayashi, Takashi Yokogawa, Takashi Yokogawa, Gota Kawai, Takuya Ueda, Kazuya Nishikawa, Kazuya Nishikawa, Kimitsuna Watanabe
J Biochem (Tokyo)   121(6) 1115-1122   1997年6月   [査読有り]
Themammalian mitochondrial tRNA(SerGCU) (mt tRNA[SerGCU]) has a unique structure in that it lacks the whole D arm. To elucidate its higher-order structure, we synthesized unmodified bovine mt tRNA(SerGCU) using T7 RNA polymerase and measured its 1...
Velinda L. Woriax, James M. Bullard, Lan Ma, Takashi Yokogawa, Linda L. Spremulli, Linda L. Spremulli
Biochim Biophys Acta   1352(1) 91-101   1997年5月   [査読有り]
Polyclonal antibodies have been prepared against both components of the bovine liver mitochondrial translational elongation factor Tu and Ts complex (EF-Tu x Ts(mt)). The antibodies against EF-Tu(mt) cross-react somewhat with Escherichia coli EF-T...
Mami Matsuo, Takashi Yokogawa, Kazuya Nishikawa, Kimitsuna Watanabe, Norihiro Okada
J Biol Chem   270(17) 10097-10104   1995年4月   [査読有り]
Two lysine isoacceptor tRNAs corresponding to the codons AAA and AAG, respectively, were isolated from squid (Loligo bleekeri), and their nucleotide sequenceswere determined. During this analysis, we discovered that the tRNA with the anticodon CUU...
Chie Takemoto, Tomohiro Koike, Takashi Yokogawa, Lisa A Benkowski, Linda L Spremulli, Takuya Ueda, Kazuya Nishikawa, Kimitsuna Watanabe
Biochimie   77 104-108   1995年1月   [査読有り]
The ability of bovine mitochondrial tRNAMet with the anticodon f5CAU (where f5C is 5-formylcytidine) to decode AUG and AUA codons was examined in a codon-dependent ribosomal binding assay. The AUG codon stimulated the binding of Met-tRNAMet to mit...
Takuya Ueda, Tsutomu Suzuki, Takashi Yokogawa, Kazuya Nishikawa, Kimitsuna Watanabe
Biochimie   76(12) 1217-1222   1994年12月   [査読有り]
In an asporogenic yeast, Candida cylindracea, codon CUG is not translated as leucine but as serine. On the basis of our recent work on the determination of the genetic code using in vitro translation systems coupled with isolation of the correspon...
Yoh-Ichi Watanabe, Gota Kawai, Takashi Yokogawa, Takashi Yokogawa, Nobuhiro Hayashi, Nobuhiro Hayashi, Yoshinori Kumazawa, Yoshinori Kumazawa, Takuya Ueda, Kazuya Nishikawa, Ichiro Hirao, Ichiro Hirao, Kin Ichiro Miura, Kin Ichiro Miura, Kimitsuna Watanabe
Nucleic Acids Res   22(24) 5378-5384   1994年12月   [査読有り]
On the basis of enzymatic probing and phylogenetic comparison, we have previously proposed that mammalian mitochondrial tRNA(sSer) (anticodon UGA) possess a slightly altered cloverleaf structure in which only one nucleotide exists between the acce...
Yuriko Komine, Makoto Kitabatake, Takashi Yokogawa, Kazuya Nishikawa, Hachiro Inokuchi
Proc Natl Acad Sci U S A   91(20) 9223-9227   1994年9月   [査読有り]
We have determined that 10Sa RNA (one of the small stable RNAs found in Escherichia coli) has an interesting structural feature: the5'end and the 3'end of 10Sa RNA can be arranged in a structure that is equivalent to a half-molecule (acceptor stem...
Jiro Moriya, Takashi Yokogawa, Kawori Wakita, Takuya Ueda, Kazuya Nishikawa, Pamela F. Crain, Takeshi Hashizume, Steven C. Pomerantz, James A. McCloskey, Gota Kawai, Nobuhiro Hayashi, Shigeyuki Yokoyama, Kimitsuna Watanabe
Biochemistry   33(8) 2234-2239   1994年5月   [査読有り]
Methionine tRNA was purified from bovine liver mitochondria, and its nucleotide sequencewas determined. The tRNA possesses only three posttranscriptionally modified nucleosides, two pseudouridines in the anticodon and T stems and a previously unkn...
Kawori Wakita, Yoh Ichi Watanabe, Takashi Yokogawa, Yoshinori Kumazawa, Yoshinori Kumazawa, Shingo Nakamura, Shingo Nakamura, Takuya Ueda, Kimitsuna Watanabe, Kazuya Nishikawa
Nucleic Acids Research   22(3) 347-353   1994年2月   [査読有り]
Bovine mitochondrial (mt) phenylalanine tRNA (tRNAPhe), which lacks the 'conserved' GG and TΨYCG sequences, was efficiently purified by the selective hybridization method using a solid phase DNA probe. The entire nucleotide sequence of the tRNA, i...
Tatsuya Niimi, Osamu Nureki, Takashi Yokogawa, Nobuhiro Hayashi, Kazuya Nishikawa, Kimitsuna Watanabe, Shigeyuki Yokoyama
Nucleosides and Nucleotides   13 1231-1237   1994年1月   [査読有り]
For Escherichia coli tRNA1/(Ile) having anticodon G34-A35-U36, two variants with substitution and/or insertion in the anticodon loop were prepared by in vitro recombinant RNA method. A variant with replacement of the N-((9-β-D-ribofuranosyl-purine...
Gota Kawail, Takashi Yokogawa, Kazuya Nishikawa, Takuya Ueda, Takeshi Hashizume, James A. McCIoskey, Shigeyuki Yokoyama, Kimitsuna Watanabe
Nucleosides and Nucleotides   13 1189-1199   1994年1月   [査読有り]
Conformational properties of a novel modified nucleoside, 5- formylcytidine (f5C), which is found at the first position of the anticodon of bovine mitochondrial tRNA(Met), were analyzed by 1H-NMR spectroscopy. f5C has a normal amino tautomeric for...
Tsutomu Suzuki, Takuya Ueda, Takashi Yokogawa, Kazuya Nishikawa, Kimitsuna Watanabe
Nucleic Acids Res   22(2) 115-123   1994年1月   [査読有り]
Five serine and three leucine isoaceptor tRNAs were purified from the asporogenic yeast Candida cylindracea, in which codon CUG is translated as serine instead of leucine, and their primary structures were determined. (more)
Shigeo Yoshionari, Tomohiro Koike, Takashi Yokogawa, Kazuya Nishikawa, Takuya Ueda, Kinichiro Miura, Kimitsuna Watanabe
FEBS Lett   338(2) 137-142   1994年1月   [査読有り]
A number of proteins functioning in mitochondria are synthesized in the cytoplasm and imported into the mitochondria via specific transport systems. In mammals, on the contrary, mitochondrial membranes have generally been considered to be impermea...
Ichiro Motoki, Takashi Yokogawa, Tsunemi Hasegawa, Kimitsuna Watanabe, Kazuya Nishikawa
Journal of Advanced Science   5(4) 85-90   1993年12月   [査読有り]
Takashi Yokogawa, Tsutomu Suzuki, Takuya Ueda, Takuya Ueda, Miki Mori, Takeshi Ohama, Yoshiyuki Kuchino, Shigeo Yoshinari, Ichiro Motoki, Kazuya Nishikawa, Syozo Osawa, Kimitsuna Watanabe, Kimitsuna Watanabe
Proc Natl Acad Sci U S A   89(16) 7408-7411   1992年8月   [査読有り]
n theasporogenic yeast Candida cylindracea, the codon CUG is read as serine instead of leucine. This is an unusual instance in which the amino acid assignment of a codon deviates from the universal code. To infer the evolutionary process of this c...
Yoshinori Kumazawa, Yoshinori Kumazawa, Takashi Yokogawa, Hiromichi Tsurui, Hiromichi Tsurui, Kin Ichiro Miura, Kin Ichiro Miura, Kimitsuna Watanabe
Nucleic Acids Res   20(9) 2223-2232   1992年5月   [査読有り]
In the course of developing a method to purify a single tRNA species efficiently, we have examined hybridization efficiencies between some tRNAs and short oligodeoxyribonucleotide probes both by the filter and solution hybridization methods withou...
Takashi Yokogawa, Yoh-Ichi Watanabe, Yoshinori Kumazawa, Takuya Ueda, Ichiro Hirao, Kin Ichiro Miura, Kimitsuna Watanabe, Kimitsuna Watanabe
Nucleic Acids Res   19(22) 6101-6105   1991年11月   [査読有り]
Bovine mitochondrial tRNASer(UCN) has been thought to have two U-U mismatches at the top of the acceptor stem, as inferred from its gene sequence. However, this unusual structure has not been confirmed at the RNA level. In the course of investigat...
Yoshinori Kumazawa, Takashi Yokogawa, Emi Hasegawa, Kin-ichiro Miura, Kimitsuna Watanabe
J Biol Chem   264(22) 13005-13011   1989年8月   [査読有り]
Bovine mitochondrial (mt)phenylalanine tRNA (tRNAPhe) was purified on a large scale using a new hybridization assay method developed by the authors. Although its melting profile suggested a loose higher order structure, presumably influenced by th...
Takashi Yokogawa, Yoshinori Kumazawa, Kin-ichiro Miura, Kimitsuna WatanabeTakashi Yokogawa, Yoshinori Kumazawa, Kin-ichiro Miura, Kimitsuna Watanabe
Nucleic Acids Res   17(7) 2623-2638   1989年4月   [査読有り]
For large scale preparation of mitochondrial tRNAs, a new hybridization assay method using synthetic oligodeoxyribonucleotide probes (16-17mer) complementary to individual tRNA sequences was developed and applied for the purification of two serine...

Misc

 
Yuichiro Nomura, Takashi Yokogawa, Shigeo Yoshinari, Yoh ichi Watanabe, Satoshi Ohno, Kazuya Nishikawa
Nucleic acids symposium series (2004)   299-300   2011年5月
At least two separate enzymes, an endonuclease and a ligase, are thought to be involved in the tRNA splicing pathway. The yeast and archaeal endonucleases acting in the first step of tRNA splicing commonly produce 2', 3'-cyclic phosphate and 5' hy...
Takashi Yokogawa, Hanim Munirah Binti Che Hassan, Yukiko Yokota, Satoshi Ohno, Kazuya Nishikawa
Nucleic acids symposium series (2004)   295-296   2011年5月
In order to create an ochre suppressor tRNA which exclusively recognizes UAA codon, we replaced the G34 at the first position of yeast tRNA(Tyr)[GPsiA] anticodon with pseudouridine34 (Psi34) by using the molecular surgery technique. This tRNA(Tyr)...
Takashi Yokogawa, Satoshi Ohno, Kazuya Nishikawa
Methods Mol Biol   607 227-242   2010年2月
An efficient method for site-selective introductionof 3-azidotyrosine into proteins has been developed. This method utilizes the yeast amber suppressor tRNA(Tyr)/mutant tyrosyl-tRNAsynthetase (Y43G) pair as the carrier of 3-azidotyrosine in an Esc...
T. Hasegawa, T. Yokogawa
Nucleic acids symposium series   7-8   2000年1月
A major proline tRNA was purified from bulk Escherichia coli A19 tRNA by affinity chromatography with a biotinylated DNA probe. Its nucleotide sequence including modified nucleotides was determined by the post-labelling technique. In order to stud...
T. Matsuki, R. Ito, T. Yokogawa, K. Nishikawa
Nucleic acids symposium series   75-76   1993年1月
A new procedure for non-radioactive labeling of the 3'-terminal -OH was developed to facilitate the sequencing works on low-molecular-weight RNAs. At first, biotinylated pCp (cytidine 3',5'-bisphosphate) was synthesized and ligated to the 3'-termi...

講演・口頭発表等

 
Click Chemistry を利用したタンパク質の部位特異的修飾法
横川 隆志(岐阜大学工学部生命工学科)
東海コンファレンス 2007 in 岐阜   2007年9月   
例えば蛍光化など"上手に"タンパク質を化学修飾すると、そのタンパク質の機能解析や他のタンパク質との相互作用解析を容易に行うことができる。通常、タンパク質内のアミノ基(Lys)やチオール基(Cys)を修飾に用いるが、LysやCysはタンパク質内に複数存在するので修飾反応がランダムに起こり、その結果得られる化学修飾タンパク質が不均一なものになってしまうという問題点があった。我々は非天然アミノ酸である3-アジドチロシン(3-N3-Tyr)をタンパク質の特異的な部位に導入し、その後、近年Click...
Methanosarcina acetivorans PylRS を利用した部位特異的リシンアナログ導入タンパク質の合成
横川 隆志(工学部生命工学科),大野 敏(工学部生命工学科),西川 一八(工学部生命工学科)
22回Archaea研究会   2009年7月   
Methanosarcina acetivoransにおけるAUAコドン解読機構
横川 隆志(工学部生命工学科),大野 敏(工学部生命工学科),西川 一八(工学部生命工学科)
23回Archaea研究会   2010年7月   
Methanosarcina acetivoransのイントロン含有tRNA前駆体と結合するタンパク質群の解析
横川 隆志(工学部生命工学科),大野 敏(工学部生命工学科),西川 一八(工学部生命工学科)
24回Archaea研究会   2011年9月   
Methanosarcina acetivoransの非標準アミノアシルtRNA合成酵素を利用した非標準アミノ酸含有タンパク質合成の試み
横川 隆志(工学部生命工学科),大野 敏(工学部生命工学科),西川 一八(工学部生命工学科)
21回Archaea研究会   2008年7月   

担当経験のある科目

 

競争的資金等の研究課題

 
tRNA スプライシング機構の特徴点抽出
日本学術振興会: 基盤C
研究期間: 2011年4月 - 2014年3月    代表者: 横川 隆志(工学部生命工学科)
文部科学省: 科学研究費補助金(基盤研究(C))
研究期間: 2011年 - 2013年    代表者: 横川 隆志
真核生物や古細菌では、タンパク質の生合成で主要な働きをするtRNAの遺伝子にイントロンが含まれている場合がある。このイントロンが除去され、成熟したtRNAが生成されるためには、RtcBという酵素がイントロンが除去された後のRNA断片を結合すると考えられるが、その際、イントロンを環状化していることが確かめられた。環状イントロンの働きは未知数であるが、環状イントロンはメタン生成古細菌 Methanosarcina acetivorans の細胞内で安定に存在することがわかった、またイントロンを...
tRNAスプライシング機構の特徴点抽出
日本学術振興会: 
研究期間: 2011年 - 2014年    代表者: 横川 隆志
tRNAスプライシング機構の特徴点抽出
日本学術振興会: 科学研究費助成事業
研究期間: 2011年 - 2013年    代表者: 横川 隆志
文部科学省: 科学研究費補助金(萌芽研究)
研究期間: 2006年 - 2007年    代表者: 横川 隆志
本年度の課題は、Leucyl/Phenylalanyl-tRNA Protein Transferaseを利用するタンパク質のN末端修飾法の修飾効率がこれまで低かった点を改善し、実際に、タンパク質のN末端にDNAを結合させることであった。これまではN末端へのアジドフェニルアラニン導入効率をいかに高めるかについて条件検討を行ってきたが、アジド基選択的な修飾法として、これまで当研究室で利用してきたStaudinger-Bertozzi ligationよりも、Crick chemistryとし...

社会貢献活動

 
岐阜大学フェア
【】  2005年9月30日 - 2005年10月2日
遺伝子ってなんだろう?
タンパク質合成系を探る
【】  岐阜新聞  岐阜新聞  2008年10月7日
シーズを探る
【】  中部経済新聞  中部経済新聞  2009年7月8日
無細胞生命科学研究会
【】  2009年11月16日 - 2009年11月17日