2020年8月
Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts
LIFE-BASEL
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- ,
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- 巻
- 10
- 号
- 8
- 開始ページ
- 154
- 終了ページ
- 154
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3390/life10080154
- 出版者・発行元
- MDPI
In this study, we aimed to evaluate the cellular response of healthy human fibroblasts induced by different types of ultra-low-fluence radiations, including gamma rays, neutrons and high linear energy transfer (LET) heavy ions. NB1RGB cells were pretreated with ultra-low-fluence radiations (similar to 0.1 cGy/7-8 h) of Cs-137 gamma rays, Am-241-Be neutrons, helium, carbon and iron ions before being exposed to an X-ray-challenging dose (1.5 Gy). Helium (LET = 2.3 keV/mu m), carbon (LET = 13.3 keV/mu m) and iron (LET = 200 keV/mu m) ions were generated with the Heavy Ion Medical Accelerator in Chiba (HIMAC), Japan. No differences in cell death-measured by colony-forming assay-were observed regardless of the radiation type applied. In contrast, mutation frequency, which was detected through cell transformation into 6-thioguanine resistant clones, was 1.9 and 4.0 times higher in cells pretreated with helium and carbon ions, respectively, compared to cells exposed to X-ray-challenging dose alone. Moreover, cells pretreated with iron ions or gamma-rays showed a mutation frequency similar to cells exposed to X-ray-challenging dose alone, while cells pretreated with neutrons had 0.15 times less mutations. These results show that cellular responses triggered by ultra-low-fluence irradiations are radiation-quality dependent. Altogether, this study shows that ultra-low-fluence irradiations with the same level as those reported in the International Space Station are capable of inducing different cellular responses, including radio-adaptive responses triggered by neutrons and genomic instability mediated by high-LET heavy ions, while electromagnetic radiations (gamma rays) seem to have no biologic impact.
- リンク情報
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- DOI
- https://doi.org/10.3390/life10080154
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/32824801
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459653
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000564609300001&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85090812528&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85090812528&origin=inward
- URL
- https://publons.com/wos-op/publon/33861311/
- ID情報
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- DOI : 10.3390/life10080154
- eISSN : 2075-1729
- ORCIDのPut Code : 79010476
- PubMed ID : 32824801
- PubMed Central 記事ID : PMC7459653
- SCOPUS ID : 85090812528
- Web of Science ID : WOS:000564609300001