論文

査読有り
2007年3月

Differential localization of aquaporin-2 and glucose transporter 4 in polarized MDCK cells

HISTOCHEMISTRY AND CELL BIOLOGY
  • Takahiro Hasegawa
  • ,
  • Toshiyuki Matsuzaki
  • ,
  • Yuki Tajika
  • ,
  • Abduxukur Ablimit
  • ,
  • Takeshi Suzuki
  • ,
  • Takeo Aoki
  • ,
  • Haruo Hagiwara
  • ,
  • Kuniaki Takata

127
3
開始ページ
233
終了ページ
241
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00418-006-0264-4
出版者・発行元
SPRINGER

Membrane water channel aquaporin-2 (AQP2) and glucose transporter 4 (GLUT4) exhibit a common feature in that they are stored in intracellular storage compartments and undergo translocation to the plasma membrane upon hormonal stimulation. We compared the intracellular localization and trafficking of AQP2 and GLUT4 in polarized Madin-Darby canine kidney cells stably transfected with human AQP2 (MDCK-hAQP2) by immunofluorescence microscopy. When expressed in MDCK-hAQP2 cells, GLUT4 and GLUT4-EGFP were predominantly localized in the perinuclear region close to and within the Golgi apparatus, similar to endogenous GLUT4 in adipocytes and myocytes. In addition, GLUT4 was occasionally seen in EEA1-positive early endosomes. AQP2, on the other hand, was sequestered in subapical Rab11-positive vesicles. In the basal state, the intracellular storage site of GLUT4 was distinct from that of AQP2. Forskolin induced translocation of AQP2 from the subapical storage vesicles to the apical plasma membrane, which did not affect GLUT4 localization. When forskolin was washed out, AQP2 was first retrieved to early endosomes from the apical plasma membrane, where it was partly colocalized with GLUT4. AQP2 was then transferred to Rab11-positive storage vesicles. These results show that AQP2 and GLUT4 share a common compartment after retrieval from the plasma membrane, but their storage compartments are distinct from each other in polarized MDCK-hAQP2 cells.

Web of Science ® 被引用回数 : 12

リンク情報
DOI
https://doi.org/10.1007/s00418-006-0264-4
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17206499
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000244187000001&DestApp=WOS_CPL

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