論文

査読有り
2020年2月11日

Dynamics of Salivary Gland AQP5 under Normal and Pathologic Conditions

International Journal of Molecular Sciences
  • Kazuo Hosoi
  • ,
  • Chenjuan Yao
  • ,
  • Takahiro Hasegawa
  • ,
  • Hiroshi Yoshimura
  • ,
  • Tetsuya Akamatsu

21
4
開始ページ
1182
終了ページ
1182
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/ijms21041182
出版者・発行元
MDPI AG

Aquaporin 5 (AQP5) plays an important role in the salivary gland function. The mRNA and protein for AQP5 are expressed in the acini from embryonic days E13-16 and E17-18, respectively and for entire postnatal days. Ligation-reopening of main excretory duct induces changes in the AQP5 level which would give an insight for mechanism of regeneration/self-duplication of acinar cells. The AQP5 level in the submandibular gland (SMG) decreases by chorda tympani denervation (CTD) via activation autophagosome, suggesting that its level in the SMG under normal condition is maintained by parasympathetic nerve. Isoproterenol (IPR), a β-adrenergic agonist, raised the levels of membrane AQP5 protein and its mRNA in the parotid gland (PG), suggesting coupling of the AQP5 dynamic and amylase secretion-restoration cycle. In the PG, lipopolysaccharide (LPS) is shown to activate mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signalings and potentially downregulate AQP5 expression via cross coupling of activator protein-1 (AP-1) and NF-κB. In most species, Ser-156 and Thr-259 of AQP5 are experimentally phosphorylated, which is enhanced by cAMP analogues and forskolin. cAMP-dependent phosphorylation of AQP5 does not seem to be markedly involved in regulation of its intracellular trafficking but seems to play a role in its constitutive expression and lateral diffusion in the cell membrane. Additionally, Ser-156 phosphorylation may be important for cancer development.

リンク情報
DOI
https://doi.org/10.3390/ijms21041182
URL
https://www.mdpi.com/1422-0067/21/4/1182/pdf

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