MISC

2005年2月

Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry

ENVIRONMENTAL TOXICOLOGY
  • MPL Chan
  • ,
  • MA Mohd

20
1
開始ページ
45
終了ページ
52
記述言語
英語
掲載種別
DOI
10.1002/tox.20076
出版者・発行元
JOHN WILEY & SONS INC

A method has been developed for the determination of trace levels of alpha-endosulfan, beta-endosulfan, endosulfan sulfate, and endosulfan diol in rat plasma and tissue samples. Endosulfan and its metabolites in the plasma samples were extracted with solid-phase extraction Chromabond-end-capped C18 cartridges and analyzed by a Shimadzu QP-5050A gas chromatograph-mass spectrometer (GCMS) with quadrupole detector in selected-ion-monitoring mode. The analysis of endosulfan and its metabolites in liver and kidney samples involved solvent extraction, Florisil solid-phase-extraction cleanup, and quantitation by GCMS. Recovery experiments for the plasma and tissue samples were conducted over concentration ranges of 10-100 ng mL(-1) and 100-1000 ng mL(-1), respectively. The method was applied to the analysis of trace levels of endosulfan and its metabolites in plasma and tissue samples collected from an animal study. Trace levels of alpha-endosulfan and beta-endosulfan in the ranges of undetectable to 3.11 mug g(-1) and undetectable to 1.19 mug g(-1), respectively, were detected in the kidney samples, whereas trace levels of endosulfan sulfate in the range of 0.02-0.22 mug g(-1) were detected in the liver samples of rats. Neither endosulfan nor its metabolites was detected in any of the plasma samples. (C) 2005 Wiley Periodicals, Inc.

リンク情報
DOI
https://doi.org/10.1002/tox.20076
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000227134500005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/tox.20076
  • ISSN : 1520-4081
  • Web of Science ID : WOS:000227134500005

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