Microbial air measurement for IPM zoning in museums is usually conducted by airborne mold samplers and agars to detect the presence of viable mold that directly poses risks for mold growth on cultural properties.Recently, bioaerosol measuring devices thatdetect biofluorescence have been developed. These devices provide results immediately with easy operation than if airborne mold samplers were to be used because there is no need for cultivation. In the present study, the availability of bioaerosol measurement for IPM zoning in a museum facility was tested. The results of measurement using a bioaerosol measuring device (Microbe SensorBM-300C, Sharp Manufacturing Systems) at the truck yard and unpacking space in Mie Prefectural Museum as well as outside showed that biofluorescence detected at each area shifted in parallel with each other and had strong correlation coefficients.The truck yard and unpacking space could be classified as the same zone which is affected by outside air containing a huge number of viable airborne mold in summer. When measured at the entrance, public space and temporary exhibition room, which are next to each other and are partitioned with automatic doors,it was found that during the hours when the museum is closed, biofluorescence detected at the entrance and public space had strong correlation coefficient,while that in the temporary exhibition room had no relationship. From the result, the temporary exhibition room could be classified as an isolated zone.On the other hand,during open hours,correlation coefficients between each area became almost comparable because visitors mix the air among these areas. In conclusion it may be said that since bioaerosol measurement can classify areas in museum facility and evaluate environment change in a period of a day, microbial air measuring method can be applied for IPM zoning in museums immediately and with moreease than airborne mold measurement.