2015年11月
Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging
NATURE PROTOCOLS
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- 巻
- 10
- 号
- 11
- 開始ページ
- 1709
- 終了ページ
- 1727
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/nprot.2015.085
- 出版者・発行元
- NATURE PUBLISHING GROUP
Here we describe a protocol for advanced CUCUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis). The CUCUBIC protocol enables simple and efficient organ clearing, rapid imaging by light-sheet microscopy and quantitative imaging analysis of multiple samples. The organ or body is cleared by immersion for 1-14 d, with the exact time required dependent on the sample type and the experimental purposes. A single imaging set can be completed in 30-60 min. Image processing and analysis can take <1 d, but it is dependent on the number of samples in the data set. The CUCUBIC clearing protocol can process multiple samples simultaneously. We previously used CUCUBIC to image whole-brain neural activities at single-cell resolution using Arc-dVenus transgenic (Tg) mice. CUCUBIC informatics calculated the Venus signal subtraction, comparing different brains at a whole-organ scale. These protocols provide a platform for organism-level systems biology by comprehensively detecting cells in a whole organ or body.
- リンク情報
- ID情報
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- DOI : 10.1038/nprot.2015.085
- ISSN : 1754-2189
- eISSN : 1750-2799
- Web of Science ID : WOS:000363472700005