2009年10月
A single circularly permuted GFP sensor for inositol-1,3,4,5-tetrakisphosphate based on a split PH domain
BIOORGANIC & MEDICINAL CHEMISTRY
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- 巻
- 17
- 号
- 20
- 開始ページ
- 7381
- 終了ページ
- 7386
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bmc.2009.08.015
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
A fluorescent sensor for the detection of inositol-1,3,4,5-tetrakisphosphate, Ins(1,3,4,5)P(4), was constructed from a split PH domain and a single circularly permuted GFP. A structure-based design was conducted to transduce a ligand-induced subtle structural perturbation of the split PH domain to an alteration in the population of the protonated and the deprotonated states of the GFP chromophore. Excitation of each distinct absorption band corresponding to the protonated or the deprotonated state of GFP resulted an increase and a decrease, respectively, in the intensity of emission spectra upon addition of Ins(1,3,4,5)P(4) to the split PH domain-based sensor. The Ins(1,3,4,5)P(4) sensor retained the ligand affinity and the selectivity of the parent PH domain, and realized the ratiometric fluorescence detection of Ins(1,3,4,5)P(4). (c) 2009 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bmc.2009.08.015
- ISSN : 0968-0896
- eISSN : 1464-3391
- Web of Science ID : WOS:000270434000032