2007年11月
An Osmium-DNA interstrand complex: Application to facile DNA methylation analysis
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
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- 巻
- 129
- 号
- 46
- 開始ページ
- 14511
- 終了ページ
- 14517
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/ja076140r
- 出版者・発行元
- AMER CHEMICAL SOC
Nucleic acids often acquire new functions by forming a variety of complexes with metal ions. Osmium, in an oxidized state, also reacts with C5-methylated pyrimidines. However, control of the sequence specificity of osmium complexation with DNA is still immature, and the value of the resulting complexes is unknown. We have designed a bipyridine-attached adenine derivative for sequence-specific osmium complexation. Sequence-specific osmium complexation was achieved by hybridization of a short DNA molecule containing this functional nucleotide to a target DNA sequence and resulted in the formation of a cross-linked structure. The interstrand cross-link clearly distinguished methylated cytosines from unmethylated cytosines and was used to quantify the degree of methylation at a specific cytosine in the genome.
- リンク情報
- ID情報
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- DOI : 10.1021/ja076140r
- ISSN : 0002-7863
- Web of Science ID : WOS:000251182000082