2007年2月
Methylcytosine-selective fluorescence quenching by osmium complexation
BIOORGANIC & MEDICINAL CHEMISTRY
- ,
- ,
- 巻
- 15
- 号
- 4
- 開始ページ
- 1615
- 終了ページ
- 1621
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bmc.2006.12.023
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
We report on the control of the emission from a fluorophore fixed on DNA using the methylcytosine-selective addition of an osmium-bipyridine complex. We have synthesized DNA modified by a microenvironment-sensitive fluorophore, 2-dimethylamino-6-acyl-naphthalene. The emission from the fluorophore tethered to a probe DNA was effectively quenched by a methylcytosine glycol-osmium-bipyridine triad, which was located in the immediate neighborhood of the fluorophore. The discrimination of the cytosine methylation status at a methylation hot spot in the p53 gene was also executed using a well-designed fluorescent DNA probe. (c) 2006 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bmc.2006.12.023
- ISSN : 0968-0896
- Web of Science ID : WOS:000243983800003