論文

査読有り
2007年2月

Methylcytosine-selective fluorescence quenching by osmium complexation

BIOORGANIC & MEDICINAL CHEMISTRY
  • Kazuo Tanaka
  • ,
  • Kazuki Tainaka
  • ,
  • Akimitsu Okamoto

15
4
開始ページ
1615
終了ページ
1621
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bmc.2006.12.023
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

We report on the control of the emission from a fluorophore fixed on DNA using the methylcytosine-selective addition of an osmium-bipyridine complex. We have synthesized DNA modified by a microenvironment-sensitive fluorophore, 2-dimethylamino-6-acyl-naphthalene. The emission from the fluorophore tethered to a probe DNA was effectively quenched by a methylcytosine glycol-osmium-bipyridine triad, which was located in the immediate neighborhood of the fluorophore. The discrimination of the cytosine methylation status at a methylation hot spot in the p53 gene was also executed using a well-designed fluorescent DNA probe. (c) 2006 Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bmc.2006.12.023
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000243983800003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bmc.2006.12.023
  • ISSN : 0968-0896
  • Web of Science ID : WOS:000243983800003

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