2010年5月
Improvement in organophosphorus hydrolase activity of cell surface-engineered yeast strain using Flo1p anchor system
Biotechnology Letters
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- 巻
- 32
- 号
- 5
- 開始ページ
- 655
- 終了ページ
- 659
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s10529-010-0204-1
- 出版者・発行元
- SPRINGER
Organophosphorus hydrolase (OPH) hydrolyzes organophosphorus esters. We constructed the yeast-displayed OPH using Flo1p anchor system. In this system, the N-terminal region of the protein was fused to Flo1p and the fusion protein was displayed on the cell surface. Hydrolytic reactions with paraoxon were carried out during 24 h of incubation of OPH-displaying cells at 30A degrees C. p-Nitrophenol produced in the reaction mixture was detected by HPLC. The strain with highest activity showed 8-fold greater OPH activity compared with cells engineered using glycosylphosphatidylinositol anchor system, and showed 20-fold greater activity than Escherichia coli using the ice nucleation protein anchor system. These results indicate that Flo1p anchor system is suitable for display of OPH in the cell surface-expression systems.
- リンク情報
- ID情報
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- DOI : 10.1007/s10529-010-0204-1
- ISSN : 0141-5492
- J-Global ID : 201002212288670897
- Web of Science ID : WOS:000276509300008