論文

査読有り 国際誌
2021年4月29日

Small-scale hypoxic cultures for monitoring the spatial reorganization of glycolytic enzymes in Saccharomyces cerevisiae.

Cell Biology International, in press
  • Yuki Yoshimura
  • ,
  • Reina Hirayama
  • ,
  • Natsuko Miura
  • ,
  • Ryotaro Utsumi
  • ,
  • Kouichi Kuroda
  • ,
  • Mitsuyoshi Ueda
  • ,
  • Michihiko Kataoka

45
8
開始ページ
1776
終了ページ
1783
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cbin.11617

At normal oxygen concentration, glycolytic enzymes are scattered in the cytoplasm of Saccharomyces cerevisiae. Under hypoxia, however, most of these enzymes, including enolase, pyruvate kinase, and phosphoglycerate mutase, spatially reorganize to form cytoplasmic foci. We tested various small-scale hypoxic culture systems and showed that enolase foci formation occurs in all the systems tested, including in liquid and on solid media. Notably, a small-scale hypoxic culture in a bench-top multi-gas incubator enabled the regulation of oxygen concentration in the media and faster foci formation. Here, we demonstrate that the foci formation of enolase starts within few hours after changing the oxygen concentration to 1% in a small-scale cultivation system. The order of foci formation by each enzyme is tightly regulated, and of the three enzymes, enolase was the fastest to respond to hypoxia. We further tested the use of the small-scale cultivation method to screen reagents that can control the spatial reorganization of enzymes under hypoxia. An AMPK inhibitor, dorsomorphin, was found to delay formation of the foci in all three glycolytic enzymes tested. These methods and results provide efficient ways to investigate the spatial reorganization of proteins under hypoxia to form a multienzyme assembly, the META body, thereby contributing to understanding and utilizing natural systems to control cellular metabolism via the spatial reorganization of enzymes.

リンク情報
DOI
https://doi.org/10.1002/cbin.11617
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33913582
ID情報
  • DOI : 10.1002/cbin.11617
  • PubMed ID : 33913582

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