2002年6月
Hepatic ischemia-reperfusion promotes liver metastasis of colon cancer
JOURNAL OF SURGICAL RESEARCH
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- 巻
- 105
- 号
- 2
- 開始ページ
- 243
- 終了ページ
- 247
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1006/jsre.2002.6356
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Background. The effects of hepatic ischemia/reperfusion (I/R) on liver metastasis have not been fully examined. We examined hepatic I/R and liver metastasis of colorectal cancer in a rat model; we also quantitated expression of E-selectin (ELAM-1) mRNA after I/R.
Materials and methods. Rats underwent 30 or 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. The number of tumor nodules on the liver surface was determined 3 weeks later. Expression of E-selectin mRNA was determined at 1, 3, and 6 h after ischemia by quantitative RT-PCR.
Results. Hepatic I/R promoted liver metastasis of RCN-H4 and induced the expression of E-selectin mRNA in both clamped and unclamped liver lobes. The number of tumor nodules and the expression of E-selectin mRNA after 60 min of ischemia was greater than that after 30 min.
Conclusions. Hepatic I/R, especially with a long duration of ischemia, induces expression of E-selectin and promotes liver metastasis of colon cancer in rats. (C) 2002 Elsevier Science (USA).
Materials and methods. Rats underwent 30 or 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. The number of tumor nodules on the liver surface was determined 3 weeks later. Expression of E-selectin mRNA was determined at 1, 3, and 6 h after ischemia by quantitative RT-PCR.
Results. Hepatic I/R promoted liver metastasis of RCN-H4 and induced the expression of E-selectin mRNA in both clamped and unclamped liver lobes. The number of tumor nodules and the expression of E-selectin mRNA after 60 min of ischemia was greater than that after 30 min.
Conclusions. Hepatic I/R, especially with a long duration of ischemia, induces expression of E-selectin and promotes liver metastasis of colon cancer in rats. (C) 2002 Elsevier Science (USA).
- リンク情報
- ID情報
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- DOI : 10.1006/jsre.2002.6356
- ISSN : 0022-4804
- Web of Science ID : WOS:000176808500024