<p>In this research, the effects on preference and visual characteristics were examined on correlations with changing parallax angle conditions in stereoscopic 3D images. Specifically, we investigated the influence of their preference decisions in 2D and 3D conditions as well as the different type of parallax angle including the negative and positive parallaxes. In experiment 1, six novel shapes excluding humans' experience were presented with five types of parallax angles (-0.5, -0.2, 0, 0.2, 0.5 degree). The experiment of paired comparison which selected one from two shapes was conducted and eye movement was measured. As a result, the negative parallax type was preferable to the positive angle, which was influenced by the absolute value of the parallax angles. In experiment 2, the time and numbers of eye fixation were measured by eye movement analysis under the same stimuli and apparatus when performing paired comparison tasks with two types of parallax angles (-0.5 and 0.5 degree). The result was that the time and numbers of eye fixation more increased during viewing negative parallax conditions than positive one.</p>