2007年9月
Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation
VIRCHOWS ARCHIV
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- 巻
- 451
- 号
- 3
- 開始ページ
- 691
- 終了ページ
- 699
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00428-007-0426-3
- 出版者・発行元
- SPRINGER
Dedifferentiated chondrosarcoma is a rare, highly malignant variant of chondrosarcoma in which a high-grade sarcoma coexists with a low-grade chondroid tumor. We herein review a case of dedifferentiated chondrosarcoma with an osteosarcoma omit component that occurred in the distal femur of a 38-year-old man. We established the cell line (NDCS-1) from a pleural effusion of the metastatic lung tumor. The cell line was characterized by a the G-banded karyotype, polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, spectral karyotyping, and reverse transcriptase PCR (RT-PCR). The tumor exhibited complex karyotypes and a high frequency of chromosomal amplication with p53 mutation. This tumor revealed an osteoblastic and chondroblastic character in vitro and in severe combined immunodeficiency mice. The expression and phosphorylation of platelet-derived growth factor receptor-beta, which seemed to play a major role in the malignant phenotype of chondrosarcoma, was confirmed by RT-PCR and Western blotting. To our knowledge, this is the first report of the establishment of a human dedifferentiated chondrosarcoma.
- リンク情報
- ID情報
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- DOI : 10.1007/s00428-007-0426-3
- ISSN : 0945-6317
- PubMed ID : 17653762
- Web of Science ID : WOS:000249124300006