論文

査読有り
2013年3月

Prevalence and molecular epidemiological characterization of antimicrobial-resistant escherichia coli isolates from Japanese black beef cattle

Journal of Food Protection
  • Shiori Yamamoto
  • ,
  • Eriko Iwabuchi
  • ,
  • Megumi Hasegawa
  • ,
  • Hidetake Esaki
  • ,
  • Masatake Muramatsu
  • ,
  • Norio Hirayama
  • ,
  • Katsuya Hirai

76
3
開始ページ
394
終了ページ
404
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.4315/0362-028X.JFP-12-273

We investigated the prevalence of antimicrobial-resistant Escherichia coli in Japanese black beef cattle from the three major production regions of Japan. We collected and examined 291 fecal samples from Japanese black beef cattle in Hokkaido, Chubu, and Kyushu. Of the 3,147 E. coli isolates, 1,397 (44.4%) were resistant to one or more antibiotics
these included 553 (39.8%) of 1,388 isolates from Hokkaido, 352 (54.4%) of 647 isolates from Chubu, and 492 (44.2%) of 1,112 isolates from Kyushu. The difference in resistance rates between the three regions was significant. The antibiotics with the highest rates of resistance were oxytetracycline and dihydrostreptomycin (35.8% each), followed by ampicillin (21.4%). Further, E. coli isolates from calves had higher resistance rates than those from growing cattle and mature cattle, and the calf isolates showed high rates of resistance to gentamicin (20.2%), enrofloxacin (9.4%), and ceftiofur (4.2%). In addition, the high degrees of similarity in the genotypes of the isolates and in the resistance patterns on each farm suggest that resistance bacteria and resistance genes were horizontally transferred. Most isolates, in each of the three regions, harbored resistance genes such as blaTEM, strA, strB, aphA1, aphAI-IAB, and catI. In contrast to the isolates from Kyushu, most of which harbored aacC2, tetB, and dfrA12, the isolates from Hokkaido and Chubu harbored a variety of resistance genes. Furthermore, the prevalence of genes for resistance to dihydrostreptomycin, gentamicin, chloramphenicol, and trimethoprim differed significantly between the regions. This is the first large-scale study describing and comparing antimicrobial-resistant bacteria from different regions in Japan. The results will contribute to improving food safety and promoting careful usage of antimicrobial agents. Copyright ©, International Association for Food Protection.

リンク情報
DOI
https://doi.org/10.4315/0362-028X.JFP-12-273
ID情報
  • DOI : 10.4315/0362-028X.JFP-12-273
  • ISSN : 0362-028X
  • SCOPUS ID : 84875514524

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