MISC

2005年4月

Gene expression and characterization of isoprene synthase from Populus alba

FEBS LETTERS
  • K Sasaki
  • ,
  • K Ohara
  • ,
  • K Yazaki

579
11
開始ページ
2514
終了ページ
2518
記述言語
英語
掲載種別
DOI
10.1016/j.febslet.2005.03.066
出版者・発行元
ELSEVIER SCIENCE BV

Isoprene synthase cDNA from Populus alha (PaIspS) was isolated by RT-PCR. This PaIspS mRNA, which was predominantly observed in the leaves, was strongly induced by heat stress and continuous light irradiation, and was substantially decreased in the dark, suggesting that isoprene emission was regulated at the transcriptional level. The subcellular localization of PaIspS protein with green fluorescent protein fusion was shown to be in plastids. PaIspS expressed in Escherichia coli was characterized enzymatically: it had an optimum pH of approximately 8.0, and an optimum temperature 40 degrees C. Its preference for divalent cations for its activity was also studied. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.febslet.2005.03.066
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000228756400045&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.febslet.2005.03.066
  • ISSN : 0014-5793
  • Web of Science ID : WOS:000228756400045

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