2006年
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
Molecular Systems Biology
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- 巻
- 2
- 号
- 開始ページ
- 2006.0008
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/msb4100050
- 出版者・発行元
- NATURE PUBLISHING GROUP
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
- リンク情報
- ID情報
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- DOI : 10.1038/msb4100050
- ISSN : 1744-4292
- Web of Science ID : WOS:000243245400009