2007年3月
Beyond the 3 ' end: experimental validation of extended transcript isoforms
Nucleic Acids Research
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- 巻
- 35
- 号
- 6
- 開始ページ
- 1947
- 終了ページ
- 1957
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/nar/gkm062
- 出版者・発行元
- OXFORD UNIV PRESS
High throughput EST and full-length cDNA sequencing have revealed extensive variations at the 3' ends of mammalian transcripts. Whether all of these changes are biologically meaningful has been the subject of controversy, as such, results may reflect in part transcription or polyadenylation leakage. We selected here a set of tandem poly(A) sites predicted from EST/cDNA sequence analysis that (i) are conserved between human and mouse, (ii) produce alternative 3' isoforms with unusual size features and (iii) are not documented in current genome databases, and we submitted these sites to experimental validation in mouse tissues. Out of 86 tested poly(A) sites from 44 genes, 84 were individually confirmed using a specially devised RT-PCR strategy. We then focused on validating the exon structure between distant tandem poly(A) sites separated by over 3 kb, and between stop codons and alternative poly(A) sites located at 4.5 kb or more, using a long-distance RT-PCR strategy. In most cases, long transcripts spanning the whole poly(A)-poly(A) or stop-poly(A) distance were detected, confirming that tandem sites were part of the same transcription unit. Given the apparent conservation of these long alternative 3' ends, different regulatory functions can be foreseen, depending on the location where transcription starts.
- リンク情報
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- DOI
- https://doi.org/10.1093/nar/gkm062
- CiNii Articles
- http://ci.nii.ac.jp/naid/80018500034
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17339231
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000246123600027&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1093/nar/gkm062
- ISSN : 0305-1048
- CiNii Articles ID : 80018500034
- PubMed ID : 17339231
- Web of Science ID : WOS:000246123600027